Re: [DIYbio] Re: Diy Segments from Thought Emporium

I don't own the Thought Emporium I am a fan of this groups Biology segments though. 

On Friday, November 30, 2018 at 5:01:40 AM UTC-8, DrBrian wrote:
really enjoying your chanell.
can you talk more about where you are and how your lab is constituted? ie community lab, academic, hobby;home?

On Fri, Nov 30, 2018 at 1:02 AM Cabalen sciences <ryangav...@gmail.com> wrote:

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Re: [DIYbio] Re: Diy Segments from Thought Emporium

really enjoying your chanell.
can you talk more about where you are and how your lab is constituted? ie community lab, academic, hobby;home?

On Fri, Nov 30, 2018 at 1:02 AM Cabalen sciences <ryangavieres123@gmail.com> wrote:

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[DIYbio] Re: Diy Segments from Thought Emporium

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[DIYbio] Finding TEV Protease (with S219V mutation) plasmid

I really need this this plasmid for my experiment. I've tried to obtain it from addgene but as I'm an individual they refuse to share it.
Anyone have this plasmid ? Thank you.

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[DIYbio] Extraction of fluorescent proteins in fish.

Is it possible to extract the fluorescent proteins from these fish? What material would be necessary?


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[DIYbio] How to Stock a DIY Biology Lab

https://www.youtube.com/watch?v=zqQ4x9rmkKg

Here is a video how to set up a DIY Lab by The Thought Emporium

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Re: [DIYbio] Rust inhibitor in water-jacketed CO2 incubator?

*coating

On Fri, Nov 23, 2018 at 8:43 AM Skyler Gordon <skgor1@gmail.com> wrote:
You could try sealing the metal with something a little more unorthodox, like two part epoxy. I'm not sure how easy it would be to apply, or if you could clean it easily after each use, or even if it would save you any money, but costing the metal to prevent rusting with sealers is a common practice in welding and metal working.

-SG
On Fri, Nov 23, 2018 at 12:27 AM <cathalgarvey@cathalgarvey.me> wrote:
Rust inhibitors for central heating systems would probably work fine? As long as they're OK for your metal type, but I imagine they're pretty versatile.


November 23, 2018 8:39 AM, "Scott" <synbiofablab@gmail.com> wrote:
We are setting up one of our stainless steel water-jacketed CO2 incubator at the lab for some BSL1 cell culture. The water in the jacket was drained about 3.5 years ago to move them. The manufacturer recommends adding rust inhibitor every two years. They don't say what the inhibitors is but the Canadian price is ridiculous at CAD$201.48 vs USD$78.39. > Biomedical Industrial Complex is alive and well - probably cost them under $20.
Any suggestion on alternate & less expensive rust inhibitors? The water needs to be neutralized.
"Fill the water jacket with 11.7 gallons (43.5 liters) of distilled water with a resistance range of 50K to 1M Ohm/cm (conductivity range of 20.0 to 1.0 uS/cm)."
"Mix 1 bag/bottle of the rust inhibitor with a gallon of water in the resistance range from 50K to 1M Ohm/cm. Drain a gallon of water from the jacket and replace it with the rust inhibitor mixture."
"High purity water (1M to 18M ohm/cm resistivity) is a very aggressive solvent and is considered slightly acidic. Ideal pH for the water in the jacket is 7. Sodium hydroxide may be used to change the pH of high purity water."
Cheers,
Scott
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Re: [DIYbio] Rust inhibitor in water-jacketed CO2 incubator?

You could try sealing the metal with something a little more unorthodox, like two part epoxy. I'm not sure how easy it would be to apply, or if you could clean it easily after each use, or even if it would save you any money, but costing the metal to prevent rusting with sealers is a common practice in welding and metal working.

-SG

On Fri, Nov 23, 2018 at 12:27 AM <cathalgarvey@cathalgarvey.me> wrote:
Rust inhibitors for central heating systems would probably work fine? As long as they're OK for your metal type, but I imagine they're pretty versatile.


November 23, 2018 8:39 AM, "Scott" <synbiofablab@gmail.com> wrote:
We are setting up one of our stainless steel water-jacketed CO2 incubator at the lab for some BSL1 cell culture. The water in the jacket was drained about 3.5 years ago to move them. The manufacturer recommends adding rust inhibitor every two years. They don't say what the inhibitors is but the Canadian price is ridiculous at CAD$201.48 vs USD$78.39. > Biomedical Industrial Complex is alive and well - probably cost them under $20.
Any suggestion on alternate & less expensive rust inhibitors? The water needs to be neutralized.
"Fill the water jacket with 11.7 gallons (43.5 liters) of distilled water with a resistance range of 50K to 1M Ohm/cm (conductivity range of 20.0 to 1.0 uS/cm)."
"Mix 1 bag/bottle of the rust inhibitor with a gallon of water in the resistance range from 50K to 1M Ohm/cm. Drain a gallon of water from the jacket and replace it with the rust inhibitor mixture."
"High purity water (1M to 18M ohm/cm resistivity) is a very aggressive solvent and is considered slightly acidic. Ideal pH for the water in the jacket is 7. Sodium hydroxide may be used to change the pH of high purity water."
Cheers,
Scott
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Re: [DIYbio] Re: Microbial Bioreactor

Hi Ravasz,

I'm not the author of this project.

I just shared the info, but I agree with you.

Every system where a surface of a instrument is exposed "constantly" to a solution containing microorganisms and nutrients there is a high probability of forming biofilms.

And probably the biofilm will interfere with the measurement (optical o electrochemical).

In some cases may be necessary to implement, somehow, a procedure to replace the solution for any other solution (oxidant, surfactant) to remove the biofilm over the surface.

Best Regards,

Markos


Em 23-11-2018 07:08, Ravasz escreveu:
Nice stuff! Really neat design.

Aren't you afraid though that the spectrometer will get clogged eventually if it keeps sampling automatically?

We are developing a bioreactor too, although smaller than your design and for a different purpose. I'll post some info on it when its ready if interested.

On Thursday, 22 November 2018 12:44:15 UTC, Markos wrote:
An open microbial bioreactor for growing cultures of about 1L:

www.hackster.io/open-bioeconomy-lab/microbial-bioreactor-d7f61b

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Re: [DIYbio] Rust inhibitor in water-jacketed CO2 incubator?

Rust inhibitors for central heating systems would probably work fine? As long as they're OK for your metal type, but I imagine they're pretty versatile.

November 23, 2018 8:39 AM, "Scott" <synbiofablab@gmail.com> wrote:
We are setting up one of our stainless steel water-jacketed CO2 incubator at the lab for some BSL1 cell culture. The water in the jacket was drained about 3.5 years ago to move them. The manufacturer recommends adding rust inhibitor every two years. They don't say what the inhibitors is but the Canadian price is ridiculous at CAD$201.48 vs USD$78.39. > Biomedical Industrial Complex is alive and well - probably cost them under $20.
Any suggestion on alternate & less expensive rust inhibitors? The water needs to be neutralized.
"Fill the water jacket with 11.7 gallons (43.5 liters) of distilled water with a resistance range of 50K to 1M Ohm/cm (conductivity range of 20.0 to 1.0 uS/cm)."
"Mix 1 bag/bottle of the rust inhibitor with a gallon of water in the resistance range from 50K to 1M Ohm/cm. Drain a gallon of water from the jacket and replace it with the rust inhibitor mixture."
"High purity water (1M to 18M ohm/cm resistivity) is a very aggressive solvent and is considered slightly acidic. Ideal pH for the water in the jacket is 7. Sodium hydroxide may be used to change the pH of high purity water."
Cheers,
Scott
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[DIYbio] Re: Microbial Bioreactor

Nice stuff! Really neat design.

Aren't you afraid though that the spectrometer will get clogged eventually if it keeps sampling automatically?

We are developing a bioreactor too, although smaller than your design and for a different purpose. I'll post some info on it when its ready if interested.

On Thursday, 22 November 2018 12:44:15 UTC, Markos wrote:
An open microbial bioreactor for growing cultures of about 1L:

www.hackster.io/open-bioeconomy-lab/microbial-bioreactor-d7f61b

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[DIYbio] Rust inhibitor in water-jacketed CO2 incubator?

We are setting up one of our stainless steel water-jacketed CO2 incubator at the lab for some BSL1 cell culture. The water in the jacket was drained about 3.5 years ago to move them. The manufacturer recommends adding rust inhibitor every two years. They don't say what the inhibitors is but the Canadian price is ridiculous at CAD$201.48 vs USD$78.39. > Biomedical Industrial Complex is alive and well - probably cost them under $20.

Any suggestion on alternate & less expensive rust inhibitors? The water needs to be neutralized.

"Fill the water jacket with 11.7 gallons (43.5 liters) of distilled water with a resistance range of 50K to 1M Ohm/cm (conductivity range of 20.0 to 1.0 uS/cm)."

"Mix 1 bag/bottle of the rust inhibitor with a gallon of water in the resistance range from 50K to 1M Ohm/cm. Drain a gallon of water from the jacket and replace it with the rust inhibitor mixture."

"High purity water (1M to 18M ohm/cm resistivity) is a very aggressive solvent and is considered slightly acidic. Ideal pH for the water in the jacket is 7. Sodium hydroxide may be used to change the pH of high purity water."

Cheers,
Scott

https://opensciencenet.org

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[DIYbio] Microbial Bioreactor

An open microbial bioreactor for growing cultures of about 1L:

www.hackster.io/open-bioeconomy-lab/microbial-bioreactor-d7f61b

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[DIYbio] Preserving Agar Art

You might find something at Amino Labs here https://amino.bio/collections They have several options. I have not used their products - just browsing their site...

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Re: [DIYbio] Preserving Agar Art

Ive seen dessication work if we leave a plate for to long or forget It. It can lead to really cool fractal patterns. Bacillus mycoides is quite cool when totally dry. 

On Wed, Nov 21, 2018, 11:29 PM Skyler Gordon <skgor1@gmail.com wrote:
Well, you could always take a nice picture of it but I would see how that has less appeal than preserving a Petri dish. If your epoxy is just on the top of the dish I could understand how that would allow the microbes to keep growing uninhibited, but as John said killing the microbes may lead to a lack of color.

You could attempt to stain some sort of cell wall or filament (actin, peptiodglycan, something there is a lot of) after some sort of heat / cold shocking to preserve both the colors and shape of your art after killing the microbes.

If you can preserve the colors after microbial death, then pouring on a two part epoxy (boat epoxies are good to buy in bulk, come in primarily clear, and can be polished easily - there is also a great company called 'ecopoxy') would still be a good idea to keep anything else from growing on the agar in the future.

If you've modified the cells the be specific colors, you could also attempt to program in some sort of cell-death mechanism. Either cell-cycle timed death or inducible death.

-SG
On Wed, Nov 21, 2018 at 6:17 AM John Griessen <john@industromatic.com> wrote:
On 11/21/18 8:36 AM, Christopher Monaco wrote:
> Hi all,
>
> Has anyone out there doing Agar Art found a reliable way to "preserve" the art once it's finished.


I've not done agar art, but have made silk screen and intaglio prints.  Learn printmaking technique and experiment with transfer
to art paper of permanent pigments or dyes that are somehow bonded to the grown cultures by color.  Art that is known to be
permanent sells a lot more than anything on cheap substrate that can never time travel.   Instead of transferring live cells, just
make a photo of your culture, image process a retouch it, and use that for photolith color layer stack up to print on fine art
paper and sell as durable art image prints in limited editions just like Warhol did so well.

Any preserved version of life will be "dull and lifeless" looking even if it contains read DNA and cell breakdown products.
Wonderfully colorful fish turn pale colored or grey when dead, same with microbes.

Creating photos with exact same size with different light filters and maybe different sprayed on solutions to change how
a culture stains could give nice contrasty grown art.  Some might work to change the staining effect when applied to the same
substrate and photographed with different filters to get nice effects of color and contrasts on different zones.  Then again use
GIMP to image process, swap colors, etc. to develop grown art for sale.

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Re: [DIYbio] Preserving Agar Art

Well, you could always take a nice picture of it but I would see how that has less appeal than preserving a Petri dish. If your epoxy is just on the top of the dish I could understand how that would allow the microbes to keep growing uninhibited, but as John said killing the microbes may lead to a lack of color.

You could attempt to stain some sort of cell wall or filament (actin, peptiodglycan, something there is a lot of) after some sort of heat / cold shocking to preserve both the colors and shape of your art after killing the microbes.

If you can preserve the colors after microbial death, then pouring on a two part epoxy (boat epoxies are good to buy in bulk, come in primarily clear, and can be polished easily - there is also a great company called 'ecopoxy') would still be a good idea to keep anything else from growing on the agar in the future.

If you've modified the cells the be specific colors, you could also attempt to program in some sort of cell-death mechanism. Either cell-cycle timed death or inducible death.

-SG

On Wed, Nov 21, 2018 at 6:17 AM John Griessen <john@industromatic.com> wrote:
On 11/21/18 8:36 AM, Christopher Monaco wrote:
> Hi all,
>
> Has anyone out there doing Agar Art found a reliable way to "preserve" the art once it's finished.


I've not done agar art, but have made silk screen and intaglio prints.  Learn printmaking technique and experiment with transfer
to art paper of permanent pigments or dyes that are somehow bonded to the grown cultures by color.  Art that is known to be
permanent sells a lot more than anything on cheap substrate that can never time travel.   Instead of transferring live cells, just
make a photo of your culture, image process a retouch it, and use that for photolith color layer stack up to print on fine art
paper and sell as durable art image prints in limited editions just like Warhol did so well.

Any preserved version of life will be "dull and lifeless" looking even if it contains read DNA and cell breakdown products.
Wonderfully colorful fish turn pale colored or grey when dead, same with microbes.

Creating photos with exact same size with different light filters and maybe different sprayed on solutions to change how
a culture stains could give nice contrasty grown art.  Some might work to change the staining effect when applied to the same
substrate and photographed with different filters to get nice effects of color and contrasts on different zones.  Then again use
GIMP to image process, swap colors, etc. to develop grown art for sale.

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Re: [DIYbio] Preserving Agar Art

On 11/21/18 8:36 AM, Christopher Monaco wrote:
> Hi all,
>
> Has anyone out there doing Agar Art found a reliable way to "preserve" the art once it's finished.


I've not done agar art, but have made silk screen and intaglio prints. Learn printmaking technique and experiment with transfer
to art paper of permanent pigments or dyes that are somehow bonded to the grown cultures by color. Art that is known to be
permanent sells a lot more than anything on cheap substrate that can never time travel. Instead of transferring live cells, just
make a photo of your culture, image process a retouch it, and use that for photolith color layer stack up to print on fine art
paper and sell as durable art image prints in limited editions just like Warhol did so well.

Any preserved version of life will be "dull and lifeless" looking even if it contains read DNA and cell breakdown products.
Wonderfully colorful fish turn pale colored or grey when dead, same with microbes.

Creating photos with exact same size with different light filters and maybe different sprayed on solutions to change how
a culture stains could give nice contrasty grown art. Some might work to change the staining effect when applied to the same
substrate and photographed with different filters to get nice effects of color and contrasts on different zones. Then again use
GIMP to image process, swap colors, etc. to develop grown art for sale.

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[DIYbio] Preserving Agar Art

Hi all,

Has anyone out there doing Agar Art found a reliable way to "preserve" the art once it's finished. We've tried simply pouring a two-part epoxy over the plates, but that doesn't stop bacterial growth. We've also tried brief UV exposure prior to pouring the epoxy but no luck either. I've read it's possible to use formalin to fix the art but I'm not sure about how to do that. Any recommendations?  

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Re: [DIYbio] Sterilizing plant hormones


Prepare in 1M NaOH, then refrigerate. You can often co-autoclave with your media, depending upon the growth regulator. The other option is to prepare in ethanol, and add that.

On Sat, Nov 17, 2018 at 2:49 AM Andreas "Mega" Stuermer <andreas.t.stuermer@gmail.com> wrote:
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

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Re: [DIYbio] Sterilizing plant hormones

Some fungal and bacterial spores are remarkably hardy, and can conceivably survive 1M NaOH. I would go ahead and filter sterilize to be safe.

On Sat, Nov 17, 2018 at 1:49 AM Andreas "Mega" Stuermer <andreas.t.stuermer@gmail.com> wrote:
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

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Re: [DIYbio] Sterilizing plant hormones


Hi Andreas!

you may ask the [PLANT-TC] group.

long time ago I made some PLANT-TC experiments in Klagenfurt
and I learned from a Netherland professional company that some
amoebe survived even hard autoclaving - they had to have it
tested by canadian specialists in order to find out.

In my naive view as a newby I think this is why many
microorganisms produce mucus - just to have some protected
encapsulation that cannot so easily be penetrated by acids and heat.

filter sterilization will not get rid of viruses but will hold back anything
larger than 15 micrometers - it will also hold back those cells
encapsulated in mucus - so my best guess is to obey the protocol.

yours, Gerald

 
_________________________________________________________________________
<experience comes shortly after it was needed>

Gerald Trost
Software Developer / Programmierer
phone: +43 (0)650 2311057
skype: gerald.trost

https://xing.com/profile/Gerald_Trost
https://linkedin.com/in/gerald-trost-57ba80ab
https://facebook.com/gerald.trost.3

_________________________________________________________________________
 
 
Sent: Saturday, November 17, 2018 at 10:49 AM
From: "Andreas \"Mega\" Stuermer" <andreas.t.stuermer@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Sterilizing plant hormones
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

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[DIYbio] Sterilizing plant hormones

Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

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[DIYbio] The Hothouse Archives: Plants, Pods and Panama Red (Free plant conference!)



November 16th-18th
FREE AND OPEN TO THE PUBLIC
School of Visual Arts
135 West 16th St
New York, NY

This conference brings together artists, architects, art historians, gardeners, philosophers and cultural critics to discuss the role of plant life in contemporary culture. 
https://www.eventbrite.com/e/the-hothouse-archives-plants-pods-and-panama-red-tickets-52101708613

"Is it possible to be a revolutionary and like flowers?" asks the artist Camille Henrot in one of her recent artworks. Continuing in that vein of wonder about the revolutionary power of plants, Taryn Simon has applied floral bouquets as a form of institutional critique. Both artists' works are examples of the political and cultural implications of plants. They are signifiers for the recurring representation of plants in contemporary arts and culture.

The symbolic meaning of plants, their relevance for religion and the metaphorical provocations in the order of knowledge, culture and political power underline the role of plants as something more than passive objects. The symbolic meaning of plants changes over time, according to cultural developments. In the origins of Western thought in particular, nature has been represented as both the embodiment of good and the epitome of evil. In Ovid's telling of humanity's golden age, the earth is an abundant source of nourishment and pleasure, while in Christianity, a snake tempts Eve into eating a forbidden apple which precipitates the fall of mankind from the Garden of Eden.

Such signification of plants and nature became intensified during the Industrial Revolution. In general, religious implications were discarded to favor images of plants and animals as savage entities. For example, the jungle in Joseph Conrad's Heart of Darkness becomes a character of its own that expresses dark and fierce forces in opposition to the concepts of light and order in European sensibilities. Human nature was characterized as a civilizing force while fauna and flora were seen as the wild and degenerative.

In the current climate, plants are undergoing radical changes due to environmental alterations and laboratory practices. From genetic selections to indoor farming, from foodstuffs and medicinal uses, plants are being re-evaluated as living entities. As sentient creatures they protect their own and engage in masquerading their identities. It has been noted that they are more like animals, only slower. They are sources of nourishment and wonder while at the same time contain healing powers and even psychoactive properties.

Organized by Suzanne Anker (Chair, BFA Fine Arts Department, School of Visual Arts, New York) and Sabine Flach (Chair, Department of Art History, University of Graz, Austria).

PROGRAM:

Friday, November 16, 2018

5:00pm - 5:45pm
Conference Registration

6:00pm - 6:30pm
Introduction
Suzanne Anker & Sabine Flach

6:30pm - 7:30pm
Keynote
Lynne van Rhijn: Herman de Vries: Shaman of Science
Moderator: Frank Gillette

7:30pm - 8:30pm
Refreshments: wine and cheese

Saturday, November 17, 2018

9:00am - 10:00am
Conference Registration and Continental Breakfast

10:00am - 12:00pm  
Panel I: Weeds and the Unrequited
Elaine Ayers: Storehouse of Nature: Nineteenth Century Exotic Flora and the Disappointment of the Tropics
EPA: The Emergent Plantocene: Weedy Vegetal Agency, Radical Embodiment, and Ruderalism X Action(ism)
Ellie Irons: The Next Epoch Seed Library
Moderator: Eva Klein

12:00pm - 1:00pm
Lunch and Bio Lab Visit

1:00pm - 2:00pm
Keynote
Melodie Yasher: Greenhouse on Mars
Moderator: Suzanne Anker

2:00pm - 2:30pm
Coffee Break and Bio Lab Visit

2:30pm - 4:00pm
Panel II: Submergence
Eva Klein: Winterwald: Concealed political fields in the depths of the forest.
Jonathan Cane: Kikuyu Grass: Decolonial Readings of the Lawn in Southern Africa
Natasha Christopher: Welkom to Johannesburg: A Tale of Two South African Cities
Moderator: Kristopher Holland

4:00pm - 4:30pm
Coffee Break and Bio Lab Visit

4:30pm - 6:30pm
Panel III: Out of the Garden
Kristopher Holland: Jean-Jacques Rousseau's Botanique: Philosophy as the Pedagogy of Romanticism & Counter-Enlightenment
Noel Anderson: The Lady's Knife: George Washington Carver Recovers the Mother Within
Sabine Flach: Unfortunately the plants did not speak Greek: Plants, Politics, Poetics
Moderator: Sabine Flach

6:30pm - 7:30pm
Psychedelic Pandemonium
Frank Gillette in conversation with Sabine Flach

7:30pm 
Cocktails and Conversation

Sunday, November 18, 2018

9:00am - 10:00am
Continental Breakfast

10:00am - 11:30pm
Panel IV: The Social Order of Plants
Karoline Walter: The Potential of Ruderal Societies and Perfectly Provisional Areas in the Works of Lois Weinberger
Teresa Castro: Queer Botanics: From Anthropomorphic Expressiveness to Plants as Queer Players in Twentieth-Century Visual Culture.
Mark Harris: Songs the Plants Taught Us: A "Bad Music" Seminar
Moderator: Sabine Flach

11:30pm - 12:30pm
Keynote 
Giovanni Aloi
Moderator: Suzanne Anker

12:30pm - 1:30pm
Lunch and Bio Lab Visit

1:30pm - 3:30pm
Panel V: Flora
Suzanne Anker: The Blue Rose
Mathias Kessler: After Nature, Coding and Reading Plant Life
Anne Percoco: Parallel Botany: The Study of Imaginary Plants
Carolyn Angleton: Propagating Desire
Heide Hatry: Not a Rose
Moderator: Suzanne Anker

3:30pm – 4:00pm
Closing Remarks
Suzanne Anker & Sabine Flach




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