Prepare in 1M NaOH, then refrigerate. You can often co-autoclave with your media, depending upon the growth regulator. The other option is to prepare in ethanol, and add that.
On Sat, Nov 17, 2018 at 2:49 AM Andreas "Mega" Stuermer <andreas.t.stuermer@gmail.com> wrote:
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.
My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?
Google was remarkably unhelpful to give a yes/no answer
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