Re: [DIYbio] Brownfield - Does it go ?

Respond to his original thread instead of making five new threads with slightly modified subject lines?  It's too hard to follow the conversation.  


On Mon, May 15, 2023, 1:29 PM Dan Kolis <dankolis@gmail.com> wrote:

Hello,

Are you going to fill in the readers here on this project ? There is a significant number of members here, who know things, have access to unique resources, etc.

You can't get mileage from that unless you cough up significant detail, for starters, on what your plan(s) are.

Where, what and when are three good things to describe most human initiatives, I've noticed.

Regards,
Daniel B. Kolis
  

 

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Re: [DIYbio] Bioremediation of brownfield soil - Controls and expectations

On 5/12/23 08:18, Dan Kolis wrote:
> I would personally immediately make pollution sets so they can be aged in situ

What is meant by pollution set?



It seems to me one needs to look at the in-situ-ation from one's chosen organisms viewpoint
to get much success in a toxin getting degraded.

As in,

How much food do they need along with the toxin to stay alive?

What ratio of normal food to toxic food causes them to eat toxins effectively? (It might be that they will only eat toxins when
normal favorite foods are low, but not low enough to starve)

Does the metabolizing of toxins give you a new toxin as reaction product?

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Re: [DIYbio] DIY Refrigerated Centrifuge



 https://joeraut.com/posts/peltier-mini-fridge/

Pelitier is commonly dismissed by HVAC ( Heating ventilating Air Con ) guys as too inefficient to compete iwth gas compression for cooling. However two things:

Coupling the semi to the load matters immensely. You can heat or cool a thermal interface and measure the temp with a themocouple and figure the thermal resistance in W/cm^2 or mm^2 etc.

So you can work with HEAT more easily, then just multiple by -1 * and design the cooling variation. Of course, just turn the voltage around the cooler heats, anyway...

So, yowling the semi is not doing enough starts with how your load gets to free air. If its lossy, thats a place to fix it. TRY COPPER NOT ALUMINUM, etc dabs of conductive goop, etc.

Also, Peltier is unusually sensitive for efficiency to % of max load. if you only use it to 10% of its designed max, its far more efficient then 80%. So *if* you want efficiency ( rare in a lab, but possible ) OVERSIZE the part and run it at a smallish percentage.

For variable temp at high precision, use PID thinking, that is approach the final temp with a duty cycle that changes of a variable part like a 555 timer of better yet a ARDINO or other few component Single board computer postage stamp part.

If the load and ambient temp is known a variable resistor or time chip is probably ok for a tolerance of +- 2 C. 

Even if you make a self controlled, closed loop you have to calibrate it occasionally before you can trust it. So really,, a simple control approach is probably ok, you should have independent temp measurements anyway for any serious work.

The URL above shows a DIY approach for a cooler.


Regs
Daniel B. Kolis



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Re: [DIYbio] DIY Refrigerated Centrifuge

Hi everyone!

Thank you very much for all your valuable inputs! Helped me so much!

With best regards,

Kavish Sirisena
IRD Genetics Sri Lanka

On Tuesday, November 1, 2022 at 12:44:20 AM UTC+5:30 dank...@gmail.com wrote:
I used Pelitier coolers for fuel systems, not life science wet lab. the Peltier 3 layer stack was pinned to a manifold which ran perhaps at a duty cycle of 50% with maybe 0.3 G of vibration continuously. As in nominally expected to run perhaps 3 years without maintenance.. This chilled Methane maybe down 45 C. Note Pelitier cooling versus expended power is very quirky.  Unlike compression chilling, its weirdly non-linear, when lightly loaded versus cell capacity, its efficient and worsens as it achieves higher loading... ex a multi layer stack will do a better job at fixed dissipation then a less or singular stack... just because the one considered is a area of a postage stamp, the same thermodynamics apply...

I see some alum or perhaps some better allow test tube holders and assoc charging stations. Creating a center of gravity for a load is by definition pretty simple.. 

Yet, actually creating anything repeatability usable is a non trivial process of trial and error. Obviously, the best way to achieve the goal a few times is go someplace else with the 'right' equipment and ... the goal is achieved.

But *if* it is easy to visualize the requirement is ongoing, especially if other temperature management requirements are achieved; ( handling simplified, lower RMS of unwanted delta T deviations, etc. ) It might be worth considering.

Managing temperature is a very very common industry requirement. Omage has a catalog the weight of a hand brick and a half with thousands of doo dads to do these things...

https://www.omega.ca/en/

50 ml seems a convenient mass to consider chilling with a rechargeable aka 'battery' one of fixture... 

ther unwanted wiggle in the centrifuge is Omega V^2 so again the question of the reasonableness or not of the notion involves extreme specification of the goals, delta T's and G's, how long the new temp must be maintained, etc....

Also, if for instance the device is rechargable on a USB 5 V port probably it can squirt back the temperature encountered. That is, instead of being an improvisation, such a device can be a normalizing aspect of utility in the science of the process itself, possibly...

Regards,
Daniel B. Kolis





On Mon, Oct 31, 2022 at 12:44 PM John Griessen <jo...@industromatic.com> wrote:
On 10/23/22 10:26, Dan Kolis wrote:
> Ive had complete success with Peltier fixtures. this $5 semi moves 8 W and has a 8 by 15 mm face...

What was it like balancing the weight and attaching wires so they don't move on a centrifuge rotor?
The 9V battery seems large for centrifuges we usually think of.  Are you using an old school centrifuge for 50 ml vials?

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