Re: [DIYbio] E. coli with pVIB not glowing, why?

When I was reading about the strain, a lot of folks complained about
recombination being possible (at least for long term maintenance of
the plasmid, or for any kind of sequence-sensitive work, like storing
a DNA library prior to sequencing)... I was actually thinking of
getting that strain and trying to select for reversions back to the
parent plasmid, using tetracycline...
https://en.wikipedia.org/wiki/PBR322

If you still have some culture around, you might try picking up some
tetracycline from a pet store and making amp+tet plates and seeing if
anything will grow.

Hard to say what happened, as I'm not too familiar with the
bioluminescent pathway and conditions for optimal light production...
but that is what comes to mind from my recent reading on pVIB and
BL21... waiting on an order myself :)

On Fri, Feb 24, 2017 at 11:24 PM, MC <markyching@gmail.com> wrote:
>> Did you grow with ampicillin?
>
>
> Yup, it came with amp media. Couldn't have been easier.
>
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--
-Nathan

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