Re: [DIYbio] E. coli with pVIB not glowing, why?

Did you grow with ampicillin?

On Fri, Feb 24, 2017 at 11:01 PM, MC <markyching@gmail.com> wrote:
> My failed pVIB E. coli experiment has been bugging me and I'm wondering if
> anyone has any ideas as to why. I got the pre-transformed kit from The Odin
> which was simple enough. My initial kit did not glow out of the box so
> Josiah was kind enough to send another one which I verified was glowing. I
> made additional LB plates and streaked them using the original glowing
> plate.
>
> Easy enough right? Except none of my plates glowed. Zero. I tried different
> temps, giving them more time to grow, etc. There was also no visible
> contamination and the cultures appeared to be growing fine. What could be
> the cause of the lack of glow?
>
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--
-Nathan

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