Yes, I've used oligos from Macrogen several times, and I have not had any problems with them. I have only used primers with basic purification (desalting) though, which may include more molecules with misplaced nucleotides, so if you are concerned about the quality in the sense of all the oligo molecules having the correct sequence, you may wish to order a higher purifcation level. Macrogen offers MOPS, PAGE and HPLC purification, but not for the smallest amount (0.025 umoles). My suggestion would be to start with ordering 0.025 umoles desalted to start with, and only upgrade if you have any problems.
-- -JP
lørdag 30. juli 2016 06.25.22 UTC+2 skrev Al.K.J følgende:
lørdag 30. juli 2016 06.25.22 UTC+2 skrev Al.K.J følgende:
Have you ever used oligos ordered from here? I'm just curious what the quality of the DNA is.
- A
On Friday, July 29, 2016 at 3:18:40 AM UTC-4, jarlemag wrote:If you are willing to pay for shipping from Korea, I can reccomend Macrogen inc (http://macrogen.com/eng/). They accept payment by credit card. There is a field for "institute/company" in their online ordering form, but you can write anything there.-JP
fredag 29. juli 2016 02.30.57 UTC+2 skrev Al.K.J følgende:
I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".
Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?
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