Not had it sequenced yet, but will be! Throat and nasopharynx as I
volunteered to be a control for a study I'm involved in. Sampling
video here, warning, contains my nose.
http://palecomic.tumblr.com/post/13012804445/im-studying-the-bacteria-living-in-or-infecting
:)
On Nov 9, 10:46 pm, Brian Degger <brian.deg...@gmail.com> wrote:
> So who out there has had their microbiome sequenced,
> I would love to one day.
> Agree with Cathal in that there is probably a lot of bacterial:bacterial
> communication/warfare going on in the gut,
> and thanks for the information about bacteria passing the gut and
> probiotics affecting the gut microbiome MikeyJ
>
>
>
>
>
>
>
>
>
> On Wed, Nov 9, 2011 at 9:34 AM, MikeyJ <mikeyj....@gmail.com> wrote:
> > Certain organisms very definitely make it past the stomach to your
> > gut, however the delivery method makes a difference. The probiotic
> > yoghurt drinks are something that one of the microbial ecology labs I
> > worked in had as a standard student project with the aim of seeing how
> > many bugs were in there and whether they were the ones that it says on
> > the packet. The overall conclusions over the years were that there
> > were frequently not very many live bugs present and that they were
> > often completely different Lactobacilli or Bifidobacteria. That's
> > significant as though the probiotic field is a muddy mess of
> > experiments, any benefits of probiotic organisms being taken into the
> > gut have been demonstrated to be strain specific.
>
> > Lyophilised bugs, above a certain CFU can definitely survive the
> > stomach (Lactobacillus has definitely been demonstrated to survive -
> >http://www.ncbi.nlm.nih.gov/pubmed?term=lactobacillus%20stomach%20sur...
> > ).
>
> > I have one good example of the effect probiotics have on the other gut
> > bugs cos it's my paper ;) (open access here
> >http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0008745)
>
> > I got poo samples from a larger study where probiotics (Lactobacillus
> > casei subsp. rhamnosus strain GG) or placebo was given to neonates
> > over the first 6 months of life from 14 babies (yeah, rubbish, I know
> > - hopefully they're going to get bigger numbers). It was done as live
> > lyophilised culture added to formula and the amount standardised. I
> > looked at microbial ecology using a 16S rRNA microarray and compared
> > the high lactobacillus poo samples with the low ones, finding that
> > lots of other bugs were also promoted in abundance when Lactobacillus
> > was high. Important to note, we didn't actually know which samples
> > were from babies that had received probiotic and which placebo, though
> > we do know it was half and half. Also, probiotics don't always
> > 'take', some people eat them and they don't establish in the gut at
> > all, whereas in other people they do. In addition you generally have
> > to keep taking them as they get flushed out once you stop dosing
> > yourself up.
>
> > There are a few good groups that are worth following up for further
> > reading, Jeff Gordon (and Ruth Ley and Peter Turnbaugh who were part
> > of his group) does lots of interesting stuffhttp://gordonlab.wustl.edu/
> > (one of my favourites is the zoo animal poo study - free
> >http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664199/?tool=pubmed)
> > including that thin and fat mouse study. In the UK the Rowett
> > Institute and Harry Flint's extended group do interesting things in a
> > different way to the poo sequencers with gut models
>
> >http://www.abdn.ac.uk/rowett/research-pages/gut-health-theme/hflint-s...
>
> > There's been an explosion of interest in this sort of stuff with gut
> > bacteria being associated with an enormous number of different health
> > and disease states, causality is much harder to assign though. Also,
> > faecal transplants make a lot more sense to me too - if in a revolting
> > way. I'm hoping it will start a craze of fad diets where supermodels
> > sell their poo for massive amounts of cash (Hollywood, we're waiting
> > for you to go first).
>
> > Cheers
>
> > Mike
>
> > On Nov 9, 7:51 am, Andrew Barney <keen...@gmail.com> wrote:
> > > I actually watched a documentary a few weeks ago while doing research
> > for a
> > > paper, and there was an interesting part about a guy who experimented on
> > > himself with some GMO bacteria that he drank in a glass of milk. The
> > > results of his unorthadox experiment showed that his bacteria never made
> > it
> > > to his gut.
>
> > > For those interested here is a link to it online. It's found at 17:30 in
> > > "Part 2 - Playing God". Actually the interesting parts are between
> > > 5:20-17:50. At about 10:00 theres a guy who was concerned about the
> > > bacteria being ingested and reaching the gut. And about 17:30 is the guy
> > > who did the experiment on himself. What's also interesting is how a lot
> > of
> > > what they are talking about and the concerns raised are almost parallel
> > to
> > > the issues and concerns facing DIYBIO today. For those interested in that
> > > part starts at about 13:00.
>
> > >http://topdocumentaryfilms.com/dna-molecule-life/
>
> > > -Andrew
>
> > > On Nov 6, 11:26 pm, Patrik <patr...@gmail.com> wrote:
>
> > > > Didn't I see some research recently showing that the organisms in
> > > > probiotic yoghurt etc. don't really make it into your gut microbiome?
> > > > I think the *idea* is good, but it may be a bit too simplistic.
> > > > Obviously, fecal transplants do work, but there you're bypassing the
> > > > stomach acids etc., and you're starting out with a gut community, not
> > > > just a yoghurt.
>
> > > > I think what may affect your gut microbiome much more is what types of
> > > > food make it into you gut, which determines what sort of nutrients and
> > > > microenvironments you're providing, and that will in turn select for
> > > > different types of organisms.
>
> > > > So perhaps yoghurt is good for you because the yoghurt bacteria have
> > > > turned the simple lactose sugars into much more complex carbohydrates,
> > > > and those in turn will encourage beneficial bacteria to grow in your
> > > > gut. But these may not necessarily be the same as the yoghurt
> > > > bacteria!
>
> > > > Same thing with fiber in your diet - we all know fiber is good for
> > > > you, but *why* exactly? Sure, there's some hypothesis about fiber
> > > > interacting with cholesterol etc. But those are hypotheses from an era
> > > > that completely ignored the role of the gut microbiome in health and
> > > > disease. I think it's far more likely that fiber selects for specific
> > > > beneficial microorganisms in you gut.
>
> > --
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> --
> ------------------------------------------
> Brian Degger
> transitlab.org
> mobile [UK]: +44 7846623934
> brian.deg...@gmail.com | skype: briandegger | twitter: @drbrian
> locale: Newcastle, UK
> ------------------------------------------
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