On 11/23/2011 01:25 PM, Simon Quellen Field wrote:
> The Beer-Lambert law will be linear between the two samples of known concentration.
> The sample will lie between those two known concentrations, and can be linearly
> interpolated easily. This can get you much higher accuracy than looking up molar
> absorptivities in a table and multiplying.
Sounds like a good method -- three cuvettes or three nanodrop-like volumes.
On 11/23/2011 01:33 PM, Nathan McCorkle wrote:
> I've never seen a sample of known
> concentration being sold
What stops you from using a standard, but unknown in absolutes, references?
You can make the low end of the range by diluting the high concentration reference.
That would give you great relative accuracy, with low cost.
JG
PS nanodrop style may not be possible in OSHW for patent reasons.
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