Actually, I have seen known concentrations sold; as ladders. If you add
up all the bands' concentrations you would have a known overall
concentration of DNA. The problem being that your ladder is suspended in
an unknown buffer; you could precipitate and resuspend, but you'd have
to be confident of your precipitation efficiency or you won't know how
much of the DNA you successfully recovered.
Any companies sell ladders in plain T.E., by any chance?
On 26/11/11 15:12, John Griessen wrote:
> On 11/23/2011 01:25 PM, Simon Quellen Field wrote:
>> The Beer-Lambert law will be linear between the two samples of known
>> concentration.
>> The sample will lie between those two known concentrations, and can be
>> linearly
>> interpolated easily. This can get you much higher accuracy than
>> looking up molar
>> absorptivities in a table and multiplying.
>
> Sounds like a good method -- three cuvettes or three nanodrop-like volumes.
>
> On 11/23/2011 01:33 PM, Nathan McCorkle wrote:
>> I've never seen a sample of known
>> concentration being sold
>
> What stops you from using a standard, but unknown in absolutes, references?
> You can make the low end of the range by diluting the high concentration
> reference.
> That would give you great relative accuracy, with low cost.
>
> JG
>
> PS nanodrop style may not be possible in OSHW for patent reasons.
>
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