Re: 16SRNA sequencing to determine differences between bacteria species

Cool. Thanks so much for this info.
I'll share results as soon as I get something interesting :)

Alexander

On Dec 1, 11:16 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
> (Specifically Patrik's response):
> On Oct 6, 12:00 am, Nathan McCorkle <nmz...@gmail.com> wrote:
>
> > I was thinking that colony PCR is really quite easy, are there
> > universal primers for 16S? I could get some in the mail in a few days
> > for not more than $5-$15... not sure if I really need that level
> > though, since I think this Prof is a "very very fair" grader.
>
> JGI typically uses universal primers 27F/1391R for bacterial 16S rRNA:http://my.jgi.doe.gov/general/protocols/SOP_16S18S_rRNA_PCR_Library_C...
> The Wikipedia page actually has a table with universal primers as
> well:http://en.wikipedia.org/wiki/16S_ribosomal_RNA#Universal_Primers
> 16S is such a common target in microbiology, that chances are somebody
> at your lab may have some primers available that you could use. Ask
> around among the grad students and postdocs...
>
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> On Thu, Dec 1, 2011 at 11:15 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
> > See this recent thread, you'll need lab access and tools to perform PCR:
>
> > How to perform bacterial ID (I am trying to determine a Bacillus species)
> >http://groups.google.com/group/diybio/browse_thread/thread/449334a66c...
>
> > On Thu, Dec 1, 2011 at 9:58 PM, Koz <kozovsk...@gmail.com> wrote:
> >> Hi ,
>
> >> I want to do a fairly simple test and determine specific species of
> >> bacteria that exist on different surfaces.
> >> The agar plate/phenotype test does not give me enough precise data, so
> >> I was wondering if there is a relatively inexpensive way to sequence
> >> microorganisms from a collection of swabs?
> >> Pretty new to this and have very limited access to basic lab equipment
> >> so supposedly I would need to send this out.
> >> Any suggestions?
>
> >> Alexander
>
> >> --
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>
> > --
> > Nathan McCorkle
> > Rochester Institute of Technology
> > College of Science, Biotechnology/Bioinformatics
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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