http://faculty.stcc.edu/rapp/BioTeach/info_on_pvib.htm
Here, that's exactly what I'm looking for!!!
It would be terrific if i could get that anyway!!!
2011/12/13 <diybio@googlegroups.com>
Group: http://groups.google.com/group/diybio/topics
- UV hood vs Flow Hood [2 Updates]
- Why Gene Patents Aren't Needed: a Proposal [1 Update]
- UV Transilluminator and mini gel electrophoresis [1 Update]
- Get a plasmide - buy? share? [1 Update]
Jeswin <phillyj101@gmail.com> Dec 12 07:37PM -0500
On Mon, Dec 12, 2011 at 5:50 PM, mad_casual <ademlookes@gmail.com> wrote:
IIRC, bacterial spores are
> about the only thing you need to worry about in a UV hood.
bacterial spores? Typo? Do you mean fungal spores?
Nathan McCorkle <nmz787@gmail.com> Dec 13 01:15AM -0500
Sent from my mobile Android device, please excuse any typographical errors.
> IIRC, bacterial spores are
> > about the only thing you need to worry about in a UV hood.
> bacterial spores? Typo? Do you mean fungal spores?
No, he (and I) mean bacterial... it should be easily googlable
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ArtifexCrastinus <deathgecko@gmail.com> Dec 12 08:38PM -0800
About the small companies being exempt from patent infringing: What
will protect the small companies from having their patents infringed
on by the large corporations? Or are you suggesting the small
companies abandon patents? My concern is that the small companies will
be constantly at odds with the giants who will suck the money out of
them through legal fees.
About nepotism and cronyism: Of course there needs to be transparency
and protocols put in place that keep conflicts of interest from
forming in the government, effectively separating the regulators from
the regulated. Laws that would keep tabs on communications between the
industry and regulators (may be very hard to implement, I admit but
it's just an idea). One of the big problems with regulators today is
that many are former (maybe even current) employees of the companies
they regulate. Maybe this could be turned around into something
positive: former employees of one company regulate that company's
competitors. Crazy enough to work?
Mackenzie Cowell <mac@diybio.org> Dec 12 05:44PM -0800
I think that is a terrific instructable. The sub-$100 UV transilluminator
is a milestone. Especially cool are the instructions on sourcing the
pro-grade UV light source for $28 and the appropriate socket and ballast
for it. (Cat# 11-2122 at http://www.fotodyne.com/content/trans_access).
I have experimented with making my own transilluminator for GelGreen and
SybrSafe stains, similar to the Pearl Biotech transilluminator instructable
you linked to. I have found that 2424 Blue Acrylic works as prefilter and
2422 Orange Acrylic works as a postfilter for those stains. I got sample
sheets from ridout plastics. Attached is a photo of a gelgreen-stained dye
sandwiched between these two acrylics illuminated underneath via a blue LED
array.
Any interest in working on a design for open-source reusable gel cassettes
for making precast gels? It would be possible to create these cassettes
directly out of the filtering acrylics.
I left similar comments for the author of the instructable.
Mac
--
+1.231.313.9062 / mac@diybio.org / @100ideas
Avery louie <inactive.e@gmail.com> Dec 12 01:52AM -0500
As far as I know, the way you get that is by asking for the creator of the
part to give it to you. That said carolina biological has a pglo plasmid
with bacterial luciferase with ampicillin resistance, if thats what you are
after.
--Avery
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