Re: Get a plasmide - buy? share?

Mega, While I have never done the prep, I would not recommend skipping
the lysozyme. From my understanding, its purpose is to break down
parts of the bacterial cell wall (namely peptidoglycan). I imagine
that eliminating it from the procedure would cause either a drastic
drop in yield or total lack of yield.

I am looking into several reagents, and I was wondering if there is
any functional difference between the various salts of EDTA available.
My guess would be that the disodium and tetrasodium salts would only
differ in the dissolved pH, and so might need additional adjusting
accordingly. However, having a calcium salt of EDTA seems
counterintuitive to me because it functions to chelate several ions in
solution, including Ca2+. Which salt is typically used in the
laboratory? Thanks!

On Dec 28, 7:14 am, Mega <masterstorm...@gmail.com> wrote:
> So my shoping list for plasmid miniprep (boiling lysis):
>
> STET-Buffer
> lysozyme (really necessary??)
> water
> isopropanol

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