Re: Get a plasmide - buy? share?

Will I need lysozyme or will the STET-buffer wwill be sufficient for a
boiling lysis??

(I'll try to get the chemicals now... Just STET, isopropanol/ethanol
and water?)

On 27 Dez., 18:13, Cathal Garvey <cathalgar...@gmail.com> wrote:
> Correction: 300mM. I blame Christmas fatigue for my unreliable literacy.
>
>
>
> Cathal Garvey <cathalgar...@gmail.com> wrote:
> >By the way, a reliable source tells me that 100mM of sodium chloride is
> >just as good as sodium acetate. As table salt is cheaper than
> >handwarmer filling, this is helpful to know.
>
> >You may want to search for 'pure' salt though, without iodine,
> >preservatives or anticaking agents, as these may affect the
> >outcome.iodine in particular would strike me as a dodgy thing to add to
> >a DNA prep, it can be pretty reactive right?
>
> >Jeswin <phillyj...@gmail.com> wrote:
>
> >>On Tue, Dec 27, 2011 at 9:55 AM, Mega <masterstorm...@gmail.com>
> >wrote:
> >>> In our university's lab there are big ammounts of 'pure' ethanol
> >>> (97%). I think isopropanol is more difficult to obtain for me.
>
> >>Isopropanol is also known as "rubbing alcohol". You can find high
> >>percentage, above 70% and maybe 80% or 90%, at your local drugstore. I
> >>would think it it is much harder to procure ethanol.
>
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