Re: Get a plasmide - buy? share?

@Ethan,

Ok, so its not that easy.... It's a pitty!

I won't need the chromosomal DNA & nuclease digesting my plasmids is
also terrible...

On 27 Dez., 20:09, Mega <masterstorm...@gmail.com> wrote:
> Will I need lysozyme or will the STET-buffer wwill be sufficient for a
> boiling lysis??
>
> (I'll try to get the chemicals now... Just STET, isopropanol/ethanol
> and water?)
>
> On 27 Dez., 18:13, Cathal Garvey <cathalgar...@gmail.com> wrote:
>
> > Correction: 300mM. I blame Christmas fatigue for my unreliable literacy.
>
> > Cathal Garvey <cathalgar...@gmail.com> wrote:
> > >By the way, a reliable source tells me that 100mM of sodium chloride is
> > >just as good as sodium acetate. As table salt is cheaper than
> > >handwarmer filling, this is helpful to know.
>
> > >You may want to search for 'pure' salt though, without iodine,
> > >preservatives or anticaking agents, as these may affect the
> > >outcome.iodine in particular would strike me as a dodgy thing to add to
> > >a DNA prep, it can be pretty reactive right?
>
> > >Jeswin <phillyj...@gmail.com> wrote:
>
> > >>On Tue, Dec 27, 2011 at 9:55 AM, Mega <masterstorm...@gmail.com>
> > >wrote:
> > >>> In our university's lab there are big ammounts of 'pure' ethanol
> > >>> (97%). I think isopropanol is more difficult to obtain for me.
>
> > >>Isopropanol is also known as "rubbing alcohol". You can find high
> > >>percentage, above 70% and maybe 80% or 90%, at your local drugstore. I
> > >>would think it it is much harder to procure ethanol.
>
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