Re: how can i check a gene is either expressed or not or over expressed after applying the drugs

Maybe a Western Blot against the protein you want to study is what you
need.
I used to do it to value the expression of some transcription factors
in prostate carcinoma and melanoma.
I know it is not a quantitative analysis and not really "real time",
but maybe it could works, i should know more about the protein :)

On 2 Dic, 09:11, Patrik <patr...@gmail.com> wrote:
> Since you're looking at a Histone Deacetylase Inhibitor, I guess you'd
> really want to check its effect on the acetylation sites of the
> target. This might be a useful reference:
>
> Probing Lysine Acetylation in Proteins
> Strategies, Limitations, and Pitfalls of in Vitro Acetyltransferase
> Assayshttp://mcponline.org/content/4/9/1226.full
>
> On Dec 1, 8:13 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
>
>
>
>
>
>
>
> > if you can easily isolate the protein, maybe you can engineer an
> > affinity tag onto the protein (though this could change natural
> > transcription levels), you can probably detect binding via electron
> > microscopy or Raman spectroscopy... if you can't do that (or don't
> > want to), maybe you could try in vitro enzyme kinetics with an
> > analogue chemical
>
> > On Thu, Dec 1, 2011 at 2:24 AM, debadutta bhoi <debadutta1...@gmail.com> wrote:
> > > i m actually doing project on a drug effect on cancer cells . the drug
> > > is actually a Histone Deacetylase Inhibitor. so when i can i monitor
> > > the drug is actually inhibiting the protein and  how can check the
> > > some particular gene is expressed.
>
> > > --
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> > --
> > Nathan McCorkle
> > Rochester Institute of Technology
> > College of Science, Biotechnology/Bioinformatics

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