Re: OpenPCR's Thermocycler - Is is reliable?

I looked at the cofactorbio website. They ran a gel of the personalPCR
sample with a commercial PCR sample. Why did they not compare a sample
from the OpenPCR? Would be nice to see the amplification from $150,
$600, and $3000 machines. Obviously, in their gel, you can see that
the band from the commercial machine is brighter than the one from the
personalPCR.

On Wed, Dec 28, 2011 at 11:16 PM, Derek <derekja@gmail.com> wrote:
> It's a really nice machine and depending on what you're trying to do
> is very sufficient. Check out http://cofactorbio.com/ for an even
> cheaper alternative if you just want to run a couple samples at a
> time. I used an OpenPCR machine for a set of classes this summer
> though and for running straightforward protocols found it in no way
> inferior to a commercial machine.
>
> --Derek
>
> On Dec 28, 7:30 pm, Jacob <lakopa.palu...@gmail.com> wrote:
>> Hello everyone,
>>
>> I am a biotechnology hobbiest located in Oregon, and I have always
>> loved biology and genetics. I was granted the amazing opportunity two
>> years ago to go to the medical school OHSU in Portland, Oregon, to
>> sequence a section of my own DNA. It was there that I learned to run a
>> thermocycler, do PCR, and load a gel tray. Ever since then, I have
>> been wishing I could get more into the field, but alas, I am still
>> just a lowly high school junior, so it may be a while. Or so I
>> thought.
>> I found out about OpenPCR a month ago, and I have been frantically
>> gathering funds since then to purchase one of their thermocyclers,
>> which is the first ever that is in my price range. I was very excited
>> to buy one, and surprisingly even more excited to trudge through their
>> 60-some page instruction book to figure out how to put the damn thing
>> together, until a friend brought up a point I shrugged off at first,
>> but now it really concerns me. "What if it doesn't actually work?"
>> Seeing as I am sinking over $600 into this machine along, not to
>> mention all the other costs associated such as reagents and pipettes,
>> I would like to know if any of you here have had experiences wit the
>> device, and how it worked for you. From what I can tell, and the
>> videos that show the device, it seems like it's a very robust and well-
>> made device. But even so, I'd like to how well it is working for any
>> of you who may have one. Is it reliable? Are the parts robust? Is the
>> cycling precise? Any kinks, flaws, or extra hacks I should know
>> about?
>>
>> Thank you!
>> --Jacob
>
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