Re: Questions about the microscope needs of the community

I'd say for checking confluence of cells you need a working distance
of about an inch, for cell identification working distance can be
sub-centimeter since you generally use glass slides (though for this
purpose I generally use 1000X magnification)

I think XY dimensions should be 4 inches or 10cm on side... I think
that would cover most petri dishes and culture tubes

On Sun, Dec 25, 2011 at 9:25 PM, Jonathan Nesser
<jonathan.nesser@gmail.com> wrote:
> I've recently been thinking about microscopes. My thought has been to
> make a kit for a webcam style microscope, inverted of course, because
> we're concerned with observing actual working cells not dead and
> mounted ones. The only thing that is really an issue is creating a
> stage for such a system (optics having already been solved for the
> most part by the guys working on stuff like the ps3 eye microscope,
> etc.) I think I've finally cracked the issue of an inverted stage that
> would be build-able with simple parts, and I'm looking for some input.
> Assuming we're only talking about working surface area, how much space
> would you need on a microscope stage? What sizes of culture flasks/
> petri dishes does everyone use? If you could, post two numbers, an
> idealized size that would fulfill your wildest dreams (if you're the
> type who dreams about this stuff, lol, I know I am), and the smallest
> space you could make due with realistically. Thanks for your
> participation, I'll try to put up some pictures/scans of design
> sketches some time soon, I've just started learning google sketch, so
> no 3d models as of yet, just pen and grid paper drawings.
>
> Jonathan Nesser
> jonathan.nesser@gmail.com
> diybioandneurosci.blogspot.com
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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