Re: UV hood vs Flow Hood

On Dec 12, 2:03 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
> On Mon, Dec 12, 2011 at 1:59 PM, Jeswin <phillyj...@gmail.com> wrote:
> > On Mon, Dec 12, 2011 at 1:47 PM, kingjacob <kingja...@gmail.com> wrote:
> >> Were these just hoods with UV lights or did they also have flow? I guess it
> >> could work to keep the workspace sterile while you aren't using it, but I
> >> always bleach everything before I get started so its a tad redundant.  I
> >> guess It depends what you are working with, but if you are doing synbio
> >> where you usually dont want mutations, i'd keep the UV away.
>
> > I asked the guy if it was laminar flow and he said it was UV hood to
> > prevent cross-contamination for their RNA work. The hood was a large
> > clear chamber able to fit on a normal lab workbench. They have the
> > wall mounted flow hoods for other jobs like cell culture.
>
> UV in this sense is just to break down the RNA, since its more labile
> than DNA anyway. I would think it would have small arm holes to
> prevent air currents as much as possible, did it?

I don't know the exact hood in question, but the UV breaks down
everything, DNA, RNA, Protein, liposomes, acrylics and polycarbonates
(I think) will photo-oxidize and 'craze'.

>
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> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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