Re: UV Transilluminator and mini gel electrophoresis

I think that is a terrific instructable.  The sub-$100 UV transilluminator is a milestone.  Especially cool are the instructions on sourcing the pro-grade UV light source for $28 and the appropriate socket and ballast for it.  (Cat# 11-2122 at http://www.fotodyne.com/content/trans_access).

I have experimented with making my own transilluminator for GelGreen and SybrSafe stains, similar to the Pearl Biotech transilluminator instructable you linked to. I have found that 2424 Blue Acrylic works as prefilter and 2422 Orange Acrylic works as a postfilter for those stains. I got sample sheets from ridout plastics.  Attached is a photo of a gelgreen-stained dye sandwiched between these two acrylics illuminated underneath via a blue LED array.

Any interest in working on a design for open-source reusable gel cassettes for making precast gels?  It would be possible to create these cassettes directly out of the filtering acrylics.

I left similar comments for the author of the instructable.

Mac


On Mon, Dec 12, 2011 at 8:22 AM, Jeswin <phillyj101@gmail.com> wrote:
Are they using black acrylic for the box? Never seen that before.

I think the blue LED Pearl box is better for a DIYbio lab. Less
dangerous equipment = safer lab. I mean, who you gonna call when you
do something stupid?

On Mon, Dec 12, 2011 at 9:15 AM, Alex Hoekstra <alex.gmu@gmail.com> wrote:
> Don't get me wrong, I love that directions for these are made
> available so that anyone so inclined can make their own, but I feel as
> though it's still out of the reach for many.  Laser-cut acrylic
> components?  Hmm.  Maybe I'll just hit up ebay...
>
> On Dec 11, 3:52 pm, Dakota Hamill <dko...@gmail.com> wrote:
>> Just got the UV Transilluminator project in my instructables weekly
>> newsletter and thought it looked pretty nice and that others might want to
>> check it out.  Turns out her other project (gel box) is pretty slick and
>> finished looking too.
>>
>> http://www.instructables.com/id/UV-Transilluminator/
>>
>> http://www.instructables.com/id/Gel-electrophoresis-system-mini/
>
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1 comments:

Sunil Patil said...

Thanks for sharing this valuable information that's really great. Xitij Instruments is leading manufacturer of lab equipments like UV transilluminator.

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