Sorry to start yet another new thread for such a small question, but I don't want to pollute other threads with off topic posts... I've been trying to understand why primer melting temperatures have to be in the 55-65C range when PCR cycles call for 94C for DNA melting... I've tried to find an explanation for this but haven't been able to... Wouldn't that result in the primers splitting off from the template DNA before you reach the extension cycle of 75C (or around there)? I'm sure I'm wrong because obviously these PCR programs work, but I don't know WHY I'm wrong, and would like to understand. Thanks again for explaining something that is probably so common knowledge that nobody feels the need to address it in writing :)
Jonathan Nesser
-- You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/3XwKiPRnTtIJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment