Re: your tips/tricks for biology lab work

For me, it's a matter of waste, not cost.

I use rubber gloves to avoid wastage, washing them and spraying with alcohol between uses. If the experiment involves nasty chemicals or if autoclaving of everything afterwards is necessary, I use disposables.

Douglas Treadwell <therealepicureanideal@gmail.com> wrote:

>Are gloves really so expensive????
>
>On Sun, Jan 29, 2012 at 1:14 AM, Mega <masterstorm123@gmail.com> wrote:
>
>> Shouldn't it be possible to sterilize gloves in the microwave??
>>
>> On 29 Jan., 09:39, Nathan McCorkle <nmz...@gmail.com> wrote:
>> > I generally reuse gloves unless I'm working in a laminar flow
>hood...
>> > because if I'm working in there, my point is to keep things really
>> > clean.
>> >
>> > Even when I work in the garage, I generally use and reuse gloves...
>> > with all the grease and dirt and metal oxides and harsh organics
>> > involved its pretty dirty... the gloves keep my hands smelling
>clean
>> > and looking the same unless I rip it while using a tool or
>something
>> > sharp.
>> >
>> > I say it depends on the risk involved, and what level of risk
>you're
>> > willing to accept. Really toxic stuff, don't play around, don't
>reuse
>> > gloves... but if you think the gloves are physically fine (no
>> > piercings, nothing melting/seeping through), and if you're careful
>> > taking them off and putting them back on, I'd say there's nothing
>to
>> > worry about.
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> > On Sat, Jan 28, 2012 at 12:55 PM, CodonAUG <elsbe...@gmail.com>
>wrote:
>> > > I wouldn't reuse gloves. The point is to protect you and the
>samples
>> > > from each other and reusing the gloves is going to defeat that
>> > > purpose.
>> >
>> > > On Jan 26, 7:36 am, Jeswin <phillyj...@gmail.com> wrote:
>> > >> Hey everyone,
>> > >> What tips, tricks, and shortcuts do you use in your lab work?
>Post
>> > >> them here. Here's 2 I recently found:
>> >
>> > >> [1] Centrifuges (I use eppendorfs) spin counter-clockwise so
>when I
>> > >> have to keep my centrifuge tube open (for eluting from spin
>columns),
>> > >> I place the lid pointing in clockwise direction so it won't
>break.
>> > >> Works great so far.
>> >
>> > >> [2] I reuse my gloves (e.g. I take them off for a bit then put
>them on
>> > >> later) so when I pull them off, they end up inside out. I was
>flipping
>> > >> them back by blowing them but I found a much safer and easier
>way.
>> > >> Collect some air in the glove, close one end, and squeeze the
>glove to
>> > >> pop the fingers out. So simple.
>> >
>> > >> Thats I remember now.
>> >
>> > > --
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>> > --
>> > Nathan McCorkle
>> > Rochester Institute of Technology
>> > College of Science, Biotechnology/Bioinformatics
>>
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