On Feb 23, 11:15 pm, rwst <r...@ark.in-berlin.de> wrote:
> Taking resveratrol as example you would first look up the pathway,
> e.g. in Metacyc or from a paper:http://metacyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-84
>
> The last 3 steps for example are catalyzed by stilbene synthase and
> resveratrol synthase.
Not quite. The last step, from 4-coumaroyl-CoA to trans-resveratrol is
catalyzed by a *single* enzyme, which is alternatively named
resveratrol synthase (in Arachis hypogaea), or stilbene synthase (in
Rheum tataricum and Vitis vinifera).
In fact, link to the Vitis enzyme is already right there on this page:
if you click on where it says "stilbene synthase (Vv)", that will take
you to this page with all the info:
http://metacyc.org/META/NEW-IMAGE?type=ENZYME-IN-PATHWAY&object=CPLX-6931
Make sure to read the Summary towards the bottom of that page, and
follow some of the reference at the bottom - that will give you a good
summary of what is known about this enzyme, and where to find teh
sequences.
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