[DIYbio] Re: Making contact to a Professor (Dr. Krichevsky)

Thank you. And it's not free to use?

""and charging something like $10 or $30 a month to contact 3-10
people ""

I assume you don't have one message for free??

On 5 Feb., 16:24, Jordan Miller <jrdn...@gmail.com> wrote:
> yes he's at linkedin I already posted the link below
>
> jordan
>
> On Feb 5, 2012, at 4:01 AM, Mega <masterstorm...@gmail.com> wrote:
>
>
>
>
>
>
>
> > I saw him on 123people and thought about registering...
> > But then I browsed his public profile and as I clicked on one article,
> > it said ''member couldn't be found''. The same happened with his other
> > articles.
>
> > I think he has abadonned his profile there....
>
> > Is anyone registred at linkedIn?? To see if his profile hasn't been
> > deleted.
>
> > _____
>
> > You said it, the plants they are now producing are very dim. So it
> > would be less problem that they're propagated because when the company
> > brings out the brightly glowing plants, consumers would like that even
> > more.
>
> > On 5 Feb., 04:54, Anselm Levskaya <levsk...@gmail.com> wrote:
> >> I don't know much about the state of this particular company, but what
> >> they're trying to do is pretty hard.
>
> >> Having used the lux operon before in bacteria, the first thing to note
> >> is that it's pretty dim, even at the maximum output levels in a
> >> healthy, dense culture.  It's much less bright than a glowstick, say.
> >> Requiring very dark adapted eyes to appreciate.
>
> >> Getting a healthy dose of photons out of a biochemical pathway
> >> requires a lot of energy.  I imagine simply putting the lux pathway
> >> into plants didn't result in a species bright enough to really impress
> >> a consumer market.  It will take a lot of work to direct more of the
> >> plants' resources into the precursors necessary to fuel the
> >> photochemical event.
>
> >> Almost all bioluminescent systems use a catalytic enzyme called a
> >> "luciferase" that activates a species specific "luciferin" with an
> >> electron-donor that ultimately ends up generating a peroxide
> >> intermediate with strained bonds whose decay is high-energy enough to
> >> cause a singlet-state excitation and quick photo-emission.
>
> >> The lux system typically only generates 10^3 photons/sec/bacterium,
> >> which can briefly result in a dim glow in a dense oxygenated culture
> >> (10^12 bacteria/mL * 10^3 = 10^15, but only when you shake them to
> >> oxygenate all the cells).  Some species of krill and fish can push
> >> 10^12 photons from their light emitting organs ~continuously.
>
> >> Note that 1 lumen is ~ 10^15 photons/sec, and a weak nightlight bulb
> >> is typically around 10^17 photons/sec, so we're talking about subtle
> >> effects here.  American fireflies can pulse brighter by controlling
> >> the photochemical reaction rate and can get up to 10^14 photons/sec.
> >> I'm not sure what the bioluminescent record holder is.  These bugs
> >> probably control brightness by constricting oxygenation of their
> >> photocytes, wherein the luciferase is found near peroxisomes and the
> >> luciferin is bunched up in cytoplasmic granules.
>
> >> Here are some general refs:http://anselmlevskaya.com/papers/bioluminescence.pdf- marine
> >> bioluminescence reviewhttp://www.photobiology.info/Viviani.html-discussion of terrestrial
> >> bioluminescence
>
> > --
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