That would be great if it doesn't 'give you a lot of
trouble' (@google translator, hope that prase is correct)
But as said, only if it doesn't make too much circumstances.
On 7 Feb., 00:32, Leon Noel <leon.a.n...@gmail.com> wrote:
> Dr. Krichevsky is a 3rd connection of mine on LinkedIn. If you haven't had
> any luck, I can try and see if I can get his info from people in the chain.
>
> Cheers,
> Leon
>
>
>
>
>
>
>
> On Mon, Feb 6, 2012 at 6:21 PM, Mackenzie Cowell <m...@diybio.org> wrote:
> > Anselm, thanks for the thoughtful writeup and back-of-the-napkin photon
> > rates. Very helpful when imagining the space of applications here.
>
> > A while ago I spent a little time reading the literature on the symbiotic
> > microbes responsible for bioluminescence in squid. I highly recommend
> > the minireview "An Exclusive Contract: Specificity in the Vibrio
> > fischeri-Euprymna scolopes Partnership":
> >http://jb.asm.org/cgi/content/short/182/7/1779. It's awesome. It tells
> > the fantastic story of how V. fischeri and a particular hawaiian squid,
> > E. scolopes, manage to meet and work together, including some detailed
> > theories about how the scolopes' special bioluminescent pockets actively
> > recruit and maintain v. fischeri cultures.
>
> > Cheers
> > mac
>
> > On Sat, Feb 4, 2012 at 7:54 PM, Anselm Levskaya <levsk...@gmail.com>wrote:
>
> >> I don't know much about the state of this particular company, but what
> >> they're trying to do is pretty hard.
>
> >> Having used the lux operon before in bacteria, the first thing to note
> >> is that it's pretty dim, even at the maximum output levels in a
> >> healthy, dense culture. It's much less bright than a glowstick, say.
> >> Requiring very dark adapted eyes to appreciate.
>
> >> Getting a healthy dose of photons out of a biochemical pathway
> >> requires a lot of energy. I imagine simply putting the lux pathway
> >> into plants didn't result in a species bright enough to really impress
> >> a consumer market. It will take a lot of work to direct more of the
> >> plants' resources into the precursors necessary to fuel the
> >> photochemical event.
>
> >> Almost all bioluminescent systems use a catalytic enzyme called a
> >> "luciferase" that activates a species specific "luciferin" with an
> >> electron-donor that ultimately ends up generating a peroxide
> >> intermediate with strained bonds whose decay is high-energy enough to
> >> cause a singlet-state excitation and quick photo-emission.
>
> >> The lux system typically only generates 10^3 photons/sec/bacterium,
> >> which can briefly result in a dim glow in a dense oxygenated culture
> >> (10^12 bacteria/mL * 10^3 = 10^15, but only when you shake them to
> >> oxygenate all the cells). Some species of krill and fish can push
> >> 10^12 photons from their light emitting organs ~continuously.
>
> >> Note that 1 lumen is ~ 10^15 photons/sec, and a weak nightlight bulb
> >> is typically around 10^17 photons/sec, so we're talking about subtle
> >> effects here. American fireflies can pulse brighter by controlling
> >> the photochemical reaction rate and can get up to 10^14 photons/sec.
> >> I'm not sure what the bioluminescent record holder is. These bugs
> >> probably control brightness by constricting oxygenation of their
> >> photocytes, wherein the luciferase is found near peroxisomes and the
> >> luciferin is bunched up in cytoplasmic granules.
>
> >> Here are some general refs:
> >>http://anselmlevskaya.com/papers/bioluminescence.pdf - marine
> >> bioluminescence review
> >>http://www.photobiology.info/Viviani.html- discussion of terrestrial
> >> bioluminescence
>
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> > --
> > +1.231.313.9062 / m...@diybio.org / @100ideas
>
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