[DIYbio] Re: Positive control for GMO foods?

Hi Brian,

That's a good suggestion thanks, I'll look into it! It'll be
interesting to take a look at their primers actually, see how closely
mine and their setups agree.

Bugs

On 1 Feb, 23:09, Brian Degger <brian.deg...@gmail.com> wrote:
> Hi Bugs
>
> Biorad has a GMO explorer kit with a refill that contains positive
> controls. Not sure how hard it is to order.
> Here is the manual for the kit www3.bio-rad.com%2Fpdf%2FBE_AN_RTGMO_v4.pdf
> and the order number for the refill is as follows
> 166-2501EDUGMO Investigator Kit Reagent Refill Pack, includes PCR
> master mix, GMO primers, plant PSII primers, GMO positive control DNA,
> PCR molecular weight ruler, Orange G loading dye
>
> Cheers
> Brian Degger
>
>
>
>
>
>
>
>
>
> On Wed, Feb 1, 2012 at 5:17 PM, Bugs <b...@binny.eu> wrote:
>
> > Hi folks,
>
> > I'm about to order some PCR primers that should let me detect the CaMV
> > 35s promoter, a viral promoter that's very commonly used in making GM
> > crops for food use. The idea, obviously, is a PCR-based test for foods
> > containing GMOs. Er, or crops that just happen to have been infected
> > by that virus, but I think it's limited to the cabbage family.
>
> > I'm struggling to think of a positive control that I can use in my
> > assay. I found a list of approved GM crops for the EU [1], but it's a
> > bit opaque. Does anyone have any pointers toward GM crops or GMO-
> > derived foods that I can buy in the UK?
>
> > Many thanks,
> > Bugs
>
> > [1]http://ec.europa.eu/food/dyna/gm_register/index_en.cfm
>
> > --
> > You received this message because you are subscribed to the Google Groups "DIYbio" group.
> > To post to this group, send email to diybio@googlegroups.com.
> > To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
> > For more options, visit this group athttp://groups.google.com/group/diybio?hl=en.
>
> --
> ------------------------------------------
> Brian Degger
> transitlab.org
> mobile [UK]: +44 7846623934
> brian.deg...@gmail.com | skype: briandegger | twitter: @drbrian
> locale: Newcastle, UK
> ------------------------------------------

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.