Since the topic "transformation in e coli" got many messages about how to make your own electroporator, I decided to open up it's discussion (then, people can identify the topic...)
We were talking about...:
>Mega
>You don't need any resistors or capacitors or ... anything?
>
>Just an ignitor, electrodes and a cuvette??????
>
>If so, Awsome!!!
>
>On 7 Feb., 17:49, Nathan McCorkle <nmz...@gmail.com> wrote:
> one or two sparks should be sufficient as long as the volts/cm is right.
>
>
> On Tue, Feb 7, 2012 at 8:14 AM, Mega <masterstorm...@gmail.com> wrote:>On 7 Feb., 17:49, Nathan McCorkle <nmz...@gmail.com> wrote:
> one or two sparks should be sufficient as long as the volts/cm is right.
>
>
> > That'll be one of my next projects... :) An electroporator....
>
> > Using an piezoelectric lighter.... Has anyone a foto of the circuit?
> > You have to push the piezoelectric botton with your hands very often,
> > or is there a circuit that transforms the voltage??
>
> > On 3 Feb., 19:55, Laureana Stelmastchuk <laure.stelmastc...@gmail.com>
> > wrote:
> >> Well, it wont answer your main questions, but could give you some great
> >> ideas...
> >> Have you ever tried electroporation? (http://en.wikipedia.org/wiki/Electroporation#Electroporators).
> >> I think electrocompetent cells are easier to prepare than the chemical
> >> ones, and it shows better transformaton rates. I have used both *E.coli *DH10B
> >> and DH5α.
> >> However, if you don't have an electroporator... I don't know, but would be
> >> cool to try to make one.
--
Laureana Stelmastchuk Benassi Fontolan
@laurestk
http://lattes.cnpq.br/0623626644642588
http://www.tumblr.com/blog/gypsyware
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