In T.E. buffer, DNA is pretty stable for months at 4c. Indeed, some schools of thought suggest that freezing is more harmful, if using buffers containing salt (like TE).
In water, far less stability is expected, but you'll probably still get transformants.
Mega <masterstorm123@gmail.com> wrote:
>Hello,
>
>I was wondering how long I could still use the plasmids I have.
>
>They were stored around 2 month in the freezer (-20°C) and now some 6
>weeks in the fridge (~7°C).
>
>Will they degrade? And, if they degrade, when only half of them will
>not be degraded (you can work with the half available, although you'll
>get smaller yields).
>
>
>Best
>
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