Re: [DIYbio] Re: DIY tube holder for vortex mixers

He's talking about bead-mediated maceration. Not just vortexing, but
vortexing with small metal beads that impact-crush everything inside.

On 02/02/12 11:16, Nathan McCorkle wrote:
> I don't see how a dremelfuge OR foam mounted on a common vortexer
> could compete with this 20 kg monster homogenizer:
> http://www.mpbio.com/product_info.php?family_key=116004500
>
> now this thing is heavy which means it can shake the hell out of the
> tubes without falling over, this means tissue will homogenize very
> quickly
>
> this doesn't mean a normal vortexer won't work, but how long will it
> take? will heat/degradation of cell components start to be an issue
> because its taking long?
>
> On Thu, Feb 2, 2012 at 3:37 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>> Despite my silence on the line, I am thinking of ways to remount a
>> dremelfuge as/on a vortex.
>>
>> It's easy to shove an offset bearing into a dremelfuge; redesigning it
>> for that wouldn't be a great problem in OpenSCAD. The problem arises
>> when you spin it by whatever shaft goes through the bearing; you still
>> need to hold the dremelfuge main body from rotating with the shaft, and
>> the way you would do so would vary from drill/driver to drill/driver.
>>
>> If you have a pre-existing vortex model (where there's just a hub that
>> oscillates without rotating) of course, it should be possible to
>> engineer the Dremelfuge simply to fit on top without too much trouble.
>> That's just a matter of replacing the dremel-adapted hub into a
>> from-beneath mount matching the vortex's fitting.
>>
>> So, in brief; vortex, easy (drill + offset skate bearing + mount, I use
>> a DIY'd one all the time). Unattended vortex, varies by design, haven't
>> bothered making one yet. Dremelfuge-that-fits-to-existing-vortex, Easy,
>> but I'd have to see pictures and precise measurements for the vortex mount.
>>
>> On 20/01/12 10:26, David McWilliam wrote:
>>> Great post. I've been looking for something like this for macerating plant
>>> material in bead beater tubes prior to DNA extraction. I've just  bought a
>>> dremelfuge which arrived today and am thinking of ways to attach it to a
>>> vortexer. The horizontal tube position would help maximise the bead
>>> vortexing although it may need something to stop the tube caps from
>>> popping. Maybe a vortexer modification of the dremelfuge - Cathal?
>>> DMcW
>>>
>>>
>>> On 20 January 2012 15:59, CodonAUG <elsbernd@gmail.com> wrote:
>>>
>>>> I wrote up a blog post about how to make a tube holder for vortex
>>>> mixers.  I needed something to hold microcentrifuge tubes for me
>>>> because I didn't want to stand there for 10 minutes with my arms
>>>> getting uncomfortably shaken.
>>>> Check it out if you are interested.
>>>>
>>>> http://cheapassscience.wordpress.com/2012/01/20/diy-tube-holder-for-vortex-mixers/
>>>>
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>>>
>>
>>
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>
>
>


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