Here's a pretty good paper on resveratrol engineering; they used a
shortcut in the pathway using L-tyrosine rather than L-phenylalanine,
which meant most cells are only three enzymes away from
trans-resveratrol synthesis.
Well, since they fused two of the enzymes (finding that it improved
yields significantly), you could say they only need two genes to make
resveratrol in any given species.
One of those species, crucially, is yeast; probably the most sensible
way to make and delivery trans-resveratrol, as it's alcohol soluble.
Alcoholic delivery may help it to get across the sublingual/buccal route
to the bloodstream (i.e., through the tissue of the mouth), which
appears to be the main way functional resveratrol reaches the blood.
More or less immediately on arriving in the digestive tract, it's
apparently conjugated to one or two compounds that seem to entirely
inhibit proper absorption.
For that reason, when I take resveratrol I usually break open a knotweed
capsule into a small amount of red wine or port, and hold it under my
tongue for a minute.
On 26/02/12 06:01, Patrik wrote:
>
>
> On Feb 23, 11:15 pm, rwst <r...@ark.in-berlin.de> wrote:
>> Taking resveratrol as example you would first look up the pathway,
>> e.g. in Metacyc or from a paper:http://metacyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-84
>>
>> The last 3 steps for example are catalyzed by stilbene synthase and
>> resveratrol synthase.
>
> Not quite. The last step, from 4-coumaroyl-CoA to trans-resveratrol is
> catalyzed by a *single* enzyme, which is alternatively named
> resveratrol synthase (in Arachis hypogaea), or stilbene synthase (in
> Rheum tataricum and Vitis vinifera).
>
> In fact, link to the Vitis enzyme is already right there on this page:
> if you click on where it says "stilbene synthase (Vv)", that will take
> you to this page with all the info:
>
> http://metacyc.org/META/NEW-IMAGE?type=ENZYME-IN-PATHWAY&object=CPLX-6931
>
> Make sure to read the Summary towards the bottom of that page, and
> follow some of the reference at the bottom - that will give you a good
> summary of what is known about this enzyme, and where to find teh
> sequences.
>
--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey
--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment