105oC should be OK. It doesn't need to be quite that high but won't hurt either. If you are seeing condensation once you open the block I would guess that you are cooling your reaction down to 4oC at the end. Most modern blocks will turn the heated lid off at that point and the differential between room temp and 4oC isn't enough to stop some condensation forming. But it is possible your lid has failed. Once you've finished with them put a couple of your PCR reactions back and run a program. If you open the lid half way through the reaction then there shouldn't be any condensation and the PCR tube lids should be too hot to touch.
Zeb
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-------- Original message --------
Subject: Re: [DIYbio] Re: how many DIYpcr machines built by DIYbio folks so far?
From: Jeswin <phillyj101@gmail.com>
To: diybio@googlegroups.com
CC:
On Sun, Feb 5, 2012 at 7:00 PM, Dakota Hamill <dkotes@gmail.com> wrote:
> Having a heated lid makes sure you don't get condensation from evaporation
> of the PCR mixture, so back in the day before heated lids people would put a
> little layer of oil on top to cut down on evaporation, then just stick in
> the pipette tip below the oil layer and pull out the final product.
>
Oh really? I still notice condensation. The lid is set to 105 degrees.
Is that too high?
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> Having a heated lid makes sure you don't get condensation from evaporation
> of the PCR mixture, so back in the day before heated lids people would put a
> little layer of oil on top to cut down on evaporation, then just stick in
> the pipette tip below the oil layer and pull out the final product.
>
Oh really? I still notice condensation. The lid is set to 105 degrees.
Is that too high?
--
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To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
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