LinkedIn is free
jordan
On Feb 6, 2012, at 3:18 PM, Mega <masterstorm123@gmail.com> wrote:
> Thank you. And it's not free to use?
>
> ""and charging something like $10 or $30 a month to contact 3-10
> people ""
>
> I assume you don't have one message for free??
>
> On 5 Feb., 16:24, Jordan Miller <jrdn...@gmail.com> wrote:
>> yes he's at linkedin I already posted the link below
>>
>> jordan
>>
>> On Feb 5, 2012, at 4:01 AM, Mega <masterstorm...@gmail.com> wrote:
>>
>>
>>
>>
>>
>>
>>
>>> I saw him on 123people and thought about registering...
>>> But then I browsed his public profile and as I clicked on one article,
>>> it said ''member couldn't be found''. The same happened with his other
>>> articles.
>>
>>> I think he has abadonned his profile there....
>>
>>> Is anyone registred at linkedIn?? To see if his profile hasn't been
>>> deleted.
>>
>>> _____
>>
>>> You said it, the plants they are now producing are very dim. So it
>>> would be less problem that they're propagated because when the company
>>> brings out the brightly glowing plants, consumers would like that even
>>> more.
>>
>>> On 5 Feb., 04:54, Anselm Levskaya <levsk...@gmail.com> wrote:
>>>> I don't know much about the state of this particular company, but what
>>>> they're trying to do is pretty hard.
>>
>>>> Having used the lux operon before in bacteria, the first thing to note
>>>> is that it's pretty dim, even at the maximum output levels in a
>>>> healthy, dense culture. It's much less bright than a glowstick, say.
>>>> Requiring very dark adapted eyes to appreciate.
>>
>>>> Getting a healthy dose of photons out of a biochemical pathway
>>>> requires a lot of energy. I imagine simply putting the lux pathway
>>>> into plants didn't result in a species bright enough to really impress
>>>> a consumer market. It will take a lot of work to direct more of the
>>>> plants' resources into the precursors necessary to fuel the
>>>> photochemical event.
>>
>>>> Almost all bioluminescent systems use a catalytic enzyme called a
>>>> "luciferase" that activates a species specific "luciferin" with an
>>>> electron-donor that ultimately ends up generating a peroxide
>>>> intermediate with strained bonds whose decay is high-energy enough to
>>>> cause a singlet-state excitation and quick photo-emission.
>>
>>>> The lux system typically only generates 10^3 photons/sec/bacterium,
>>>> which can briefly result in a dim glow in a dense oxygenated culture
>>>> (10^12 bacteria/mL * 10^3 = 10^15, but only when you shake them to
>>>> oxygenate all the cells). Some species of krill and fish can push
>>>> 10^12 photons from their light emitting organs ~continuously.
>>
>>>> Note that 1 lumen is ~ 10^15 photons/sec, and a weak nightlight bulb
>>>> is typically around 10^17 photons/sec, so we're talking about subtle
>>>> effects here. American fireflies can pulse brighter by controlling
>>>> the photochemical reaction rate and can get up to 10^14 photons/sec.
>>>> I'm not sure what the bioluminescent record holder is. These bugs
>>>> probably control brightness by constricting oxygenation of their
>>>> photocytes, wherein the luciferase is found near peroxisomes and the
>>>> luciferin is bunched up in cytoplasmic granules.
>>
>>>> Here are some general refs:http://anselmlevskaya.com/papers/bioluminescence.pdf- marine
>>>> bioluminescence reviewhttp://www.photobiology.info/Viviani.html-discussion of terrestrial
>>>> bioluminescence
>>
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