[DIYbio] How long are sperm viable in-vitro

I'm looking for the length of time until death of spermatozoa isolated
from ejaculate, incubated in mammalian cell culture media. Even a
paper showing time post-ejaculation vs in-vitro fertilization (IVF)
success would be good. I basically want to know how long these suckers
can stay alive outside the body, though I'm pretty sure they don't
divide.

This could lead to things like glowing ejaculate in a tube... or
potentially @home IVF. A lot more equipment would need developed for
the latter to be @home... but heck, let's not be thought police on
far-out ideas.


This gives some simple vitality assays, and isolation methods, but
doesn't really show a time vs vitality curve
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006446

This is just kind of weird... I wonder what would happen if they implanted:
http://onlinelibrary.wiley.com/doi/10.1002/mrd.1120160108/abstract

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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