I am putting together the reagants for a plasmid miniprep kit for myself and had a simple question if anybody out there has done this. Two of the solutions require guanidium HCl (http://www.roningenetics.org/Protocols.html, about 80% of the way down the page under the heading "Solutions for plasmid minipreps", buffer N3 and buffer PB, 4M and 5M guanidium HCl, respectively). Having always bought commercial kits before, I was wondering if these two solutions really needed autoclaving. I suspect not since guanidium is a strong protein denaturant so it seems highly unlikely anything, including DNases, would survive a 4 or 5M solution of this. I will test my kit without autoclaving first, but any suggestions would be welcome.
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/4ZxwUAVdL1oJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment