[DIYbio] Re: Fluorescent Microscope Incubator DIY

Have you heard of Hibernate-E?  Suppsoedly its media formulated for growing cells without CO2 (specifically neural tissue but maybe others too).

https://products.invitrogen.com/ivgn/product/A1247601?ICID==%3Dsearch-product

In regards to your concerns over putting microscopes into humid environments, contact the manufacturer.  Many microscopes are designed to tolerate moisture and temperature for very long periods.  Some more food for thought, fluctuations in ambient temperature with time during a time lapse can changes the focus and image quality but this is prevented either with an incubated microscope or special covers for the objectives.

Depending on how well funded your project is, you could buy pressured air tanks (or mix the tanks in line with equipment) that have the 5% Co2, 95% air.  Then blow that over your cells directly during the long term study (one air tank will last 2-3 days of constant flow over cells I think).  Just bubble the air line through warm water and then back through a tube to the cells to keep the moisture at a good level.

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