Since you mentioned you had a laser cutter check out the openspectrometer.com guys and in particular the third video on their site. They show a pretty clever way to make 'cuvettes' or simple holders for the spectrometer, but the design would lend itself well to being scaled up a little bit to make a cell culture holder that you would mate to a microscope since it has two 'optical surfaces'. Further the C02 could be pipe in via larger holes then what they show in the movie.
Chris
On Tuesday, March 6, 2012 12:14:24 PM UTC-5, Koz wrote:
That is a very interesting sketch and very similar to what I was
thinking.
My thought was to use the regulation from the main incubator and pipe
the environment in.
However I was worried that if there are any cold spots, it would
result in condensation and a ruined image.
I see the circular flow is meant to avoid this.
Thanks, I'll start prototyping some things and see how it goes.
On Mar 6, 10:39 am, John Griessen <j...@industromatic.com> wrote:
> On 03/06/2012 01:16 AM, Koz wrote:
>
> > Timelapse will last about 60 hours, and the cells(murine fibroblasts)
> > need to be in incubation + CO2, so placing them on the stage for the
> > duration is not going to work.
>
> How about an incubator "slab" with support gear outside connected by tubes
> to keep it warm/humid, and a second chamber around the main one to make
> a double pane window to lessen chances of condensation?
>
> See sketch: http://ecosensory.com/diybio/stage_incubator-3.jpghttp:// ecosensory.com/diybio/stage_ incubator-3.gif
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