This is really DNA extraction, though there is probably a significant
amount of other detritus mixed in with it. If you include a protease
in the procedure, that should yield more pure DNA. Human chromosomal
DNA (assuming it is in tact) ranges from about 50-300 megabase pairs.
Plasmid DNA tops out somewhere around 30 kilobase pairs, so there is
significantly larger pieces of DNA in eukaryotic cells than plasmid
DNA in prokaryotes. The size of the pieces also affect how quickly
they would settle under centrifugation.
DNA extraction from fruit is a pretty popular demonstration
experiment. I have a little jar of cavendish banana (Musa acuminata
AAA) DNA that I keep on my desk. The yield of DNA from bananas was
surprisingly high, which I think may be a result of triploidy of the
particular strain.
On Mar 3, 6:39 pm, Jeswin <phillyj...@gmail.com> wrote:
> Honestly, is this really DNA extraction or something else. Is it
> because of the structure of human DNA (vs plasmid DNA) that its so
> easy to do this? When I extract plasmid DNA in isopropanol, we spin it
> at 6000rcf for 50 minutes at 4C (or if you got faster centrifuge, less
> time).
>
>
>
>
>
>
>
> On Sat, Mar 3, 2012 at 5:57 PM, Randy <bebo...@gmail.com> wrote:
> > I haven't tried it, although the video is fun to watch.
>
> >http://www.youtube.com/watch?v=DaaRrR-ZHP4
>
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