I've made my own buffers before. I followed the formulation on http://openwetware.org/wiki/Qiagen_Buffers
I believe I filtered those solutions rather than autoclaving since I had a filter already set up. I doubt that either was necessary, though, since as you say the guanidium is at such high concentrations.
--Derek
On Sunday, 25 March 2012 16:50:46 UTC-7, Tom Randall wrote:
I am putting together the reagants for a plasmid miniprep kit for myself and had a simple question if anybody out there has done this. Two of the solutions require guanidium HCl (http://www.roningenetics.org/Protocols.html, about 80% of the way down the page under the heading "Solutions for plasmid minipreps", buffer N3 and buffer PB, 4M and 5M guanidium HCl, respectively). Having always bought commercial kits before, I was wondering if these two solutions really needed autoclaving. I suspect not since guanidium is a strong protein denaturant so it seems highly unlikely anything, including DNases, would survive a 4 or 5M solution of this. I will test my kit without autoclaving first, but any suggestions would be welcome.--
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