Is this really sterile/aseptic?
Because why would university use the autoclavator if it's that easy?
Have you never had significant contamination using this technique??
On 8 Mrz., 17:47, Jeswin <phillyj...@gmail.com> wrote:
> On Thu, Mar 8, 2012 at 10:20 AM, Mega <masterstorm...@gmail.com> wrote:
> > So I make Lb-amp agar and plate the lawn on it. at university I was
> > shown to autoclave the agar before pouring plates. i can either do
> > that in the pressure cooker or just leave it in the microwave for 10
> > mins (?)
>
> For LB-agar, I boil some water in the flask in the microwave, which is
> 2 to 3 minutes. That kills anything in there. Then I let it cool and
> add my purified H20 and LB-Agar, and boil in microwave until it is all
> dissolved.
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