Re: Back to Transformation! (Was Re: [DIYbio] Re: Which microscope?)

there is a lawn of bacteria on the plates.

I didn't look at them at night because they are in the university
lab.


But would it be posssible to see them glowing if only 1/1000 of them
glows and maybe the low amp has killed 1/2 of bacteria? ->1/500

I can either put amp-water on them, to kill the lawn now. Only
transformants should survie and form new colonies??
Or I make a new amp-plate and put the lawn on it?

On 7 Mrz., 02:17, Avery louie <inactiv...@gmail.com> wrote:
> Hey Mega,
>
> how did it go?  was there any plasmid uptake even without much antibiotic?
>
> --Avery
>
>
>
>
>
>
>
> On Mon, Mar 5, 2012 at 9:50 AM, Mega <masterstorm...@gmail.com> wrote:
> > Something positive: Last october there was a 3-day course of biology
> > with basics like PCR, Plasmid Preparation, Transformation. Only a bit
> > more than 1 hour away!!! (it was kinda hidden, only 12 members)
>
> > I've spotted an article in the web
> >http://service.salzburg.gv.at/lkorrj/Index?cmd=detail_ind&nachrid=47840
> > and asked via email if this year there'll be again such a course. (No
> > answer so far)
>
> > --
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