On Thu, Mar 22, 2012 at 5:15 AM, Pat <eleten@gmail.com> wrote:
> @philly Are you using BLASTN or Primer-BLAST?
>
I guess BLASTN. I haven't heard of Primer-BLAST until now.
The HBV genome is highly polymorphic so getting a primer that will
bind to most of the DNA fragments is a bit hard to make. I'm still new
to this so maybe it is easier for others.
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