"A third reason for error applies primarily to unverified databases such as GenBank, which accept any linked name and sequence that is sent to them. In the 1995 investigation of GenBank sequences, a comparison of sequences for the presumed same species showed that almost 20% had more than 2% variability, indicating that many genetically different strains were being deposited under the same species name (18). On some occasions, the name associated with a given sequence may not be correct due to poor phenotypic descriptions or faulty testing."
Here was the article I had mentioned way up above if anyone is still interested
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC523561/?tool=pubmed
It should be free, was an interesting read, and although maybe not fully related, it did delve into assigning phylogeny of bacteria using 16s sequences and the problems associated with it
and this was the commercial database they were talking about http://www.appliedbiosystems.com/absite/us/en/home/support/software/dna-sequencing/microseq-id-systems.html but I can't tell if it's free or not it keeps giving this computer I am using an unzip error or something, and since it looks like it is only 16s and a fungal ID'er...not of much use. Oh well, something to be aware of might be useful in the future. Good luck on your quest
-Dakota
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