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Get a free science project every week! "http://scitoys.com/newsletter.html"Excellent!
That would seem to solve the humidity problem as well -- keep everything
soaking wet.
Since the medium will presumably need some oxygen for the cells, you might
replace the syringe pump with an aquarium air pump bubbling air into a jar
of the medium. The air pressure would then cause the medium to flow through
the incubator. Keeping a large jar of medium at a constant temperature is
easier than keeping a stage incubator constant, and could probably be done
with an aquarium heater and a water bath.
PDMS is widely used for microfluidics, but this particular application is not all
that micro, and I really liked the double-stick tape method, as it is quick and
very DIY accessible. Stick the tape on the slide, and use an Xacto knife to cut
the channels and chambers, then apply the cover slip. Was that Nathan's idea,
for the spectrometer cuvettes? I love it.
On Tue, Mar 6, 2012 at 7:32 PM, AKS <aungkyas@gmail.com> wrote:
Hi
I think what you are trying to do is to culture some cells and I assume
you are working on mammalian cells as you need CO2 buffer. I think if
you have access to syringe pump with programmable syringe then you
can get rid of CO2 buffer as long as you can warm up the cell culture
medium in syringe pump to be at 37.5 Degree Celcius. CO2 buffer is
for pH control and if you can set up a continuous cell culture medium
renewal, that buffer may be redundant. If you have access to microfluidics
then it is easy to fabricate PDMS (Silicone polymer) to develop micro-cell
culture/perfusion chips and they are very cheap to make. I hope this helps.
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