A little late to the party so I am building on what others have said. You obviously have a scope and an incubator. So take a tissue culture flask and rig it with a tube in and out. Run those to another flask in the incubator. Add a peristaltic pump and then you can recirculate gassed media from the incubator into the microscope flask. Make sure the scope flask is completely full and you won't have any condensation problems. Now the scope only needs to be next to the incubator :-)
Zeb
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-------- Original message --------
Subject: Re: [DIYbio] Fluorescent Microscope Incubator DIY
From: Koz <kozovski.a@gmail.com>
To: diybio@googlegroups.com
CC:
That is interesting.
Thanks for the link!
On Tuesday, March 6, 2012 11:20:44 AM UTC-5, Simon Field wrote:
-- On Tuesday, March 6, 2012 11:20:44 AM UTC-5, Simon Field wrote:
Something like this?It appears you can buy the parts separately, and build what you need:The temperature controller and gas supplies can be DIY without much trouble.-----Get a free science project every week! "http://scitoys.com/newsletter.html "
On Tue, Mar 6, 2012 at 7:39 AM, John Griessen <john@industromatic.com> wrote:On 03/06/2012 01:16 AM, Koz wrote:How about an incubator "slab" with support gear outside connected by tubes
Timelapse will last about 60 hours, and the cells(murine fibroblasts)
need to be in incubation + CO2, so placing them on the stage for the
duration is not going to work.
to keep it warm/humid, and a second chamber around the main one to make
a double pane window to lessen chances of condensation?
See sketch: http://ecosensory.com/diybio/stage_incubator-3.jpg
http://ecosensory.com/diybio/stage_incubator-3.gif
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