On Mon, Mar 26, 2012 at 7:21 PM, Cory Tobin <cory.tobin@gmail.com> wrote:
>
> You can use diatomaceous earth (sold at pool supply stores). Instead
> of packing them into a column and centrifuging the liquid through the
> column you add the diatomaceous earth powder to the solution, vortex
> to suspend it, then quickly centrifuge to pellet the diatomaceous
> earth, then either pour or pipette away the supernatant. As described
> here:
>
> Boom, R., Sol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-van
> Dillen, P. M., & van Der Noordaa, J. (1990). Rapid and simple method
> for purification of nucleic acids. Journal of Clinical Microbiology,
> 28(3), 495-503. Retrieved from
> http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269651&tool=pmcentrez&rendertype=abstract
>
Cool. I wonder what the efficiency difference is between this and
commercial columns.
--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment