Thank you for all the answers so far everyone.
I'm hoping to work with a strain of archaea native to human gut flora. Alas I've been advised that they are not particularly robust. If possible I'd like to keep the link to human microbes.
Do the platinum wires help eliminate the presence of oxygen (forgive my ignorance on these matters)
Generating methane is easy:Methanogens are pretty robust -- you won't have to worry a lot about their survivalin a slurry of animal waste and straw. Why you would pick a "particularly difficultto culture strain" for this project is a mystery only you can shed light upon.So, while I do in fact sell platinum coated nickel wire on my web site for a priceyou will find hard to beat elsewhere, I would recommend simply using the bacteriathat is already in the manure, and not bothering with platinum at all.-------Get a free science project every week! "http://scitoys.com/newsletter.html"
On Wed, Mar 21, 2012 at 2:19 PM, Mega <masterstorm123@gmail.com> wrote:It may be that my native language is not English, but I don't see the problem...
If the (former marmelade) jar needs to contain no air, then flood it by the gas of a cigarette lighter.
Platinum wire and other metal wires are often sold e.g. on ebay. Surely, platinum is expensive. But the wires are quite affordable, as those are just a few gramms. And maybe just the surface is platinum - those are even cheaper. Make a lattice with small gaps, for filtering the air?
Ore take a lattice of iron and galvanize it with platinum (less work with making the latice, cheaper. If you have the chemicals)
That's just my idea... But I don't know how obligate anaerobe those are....
Am Mittwoch, 21. März 2012 21:46:39 UTC+1 schrieb Stephen Fortune:To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/TtrFDOv4xLIJ.--Greetings one and all
I'm here, figurative cap in hand, to ask a favour of your expertise.
I need to use an anaerobic jar to grow a particularly difficult to culture strain of methanogen. They are completely anaerobic.
It's for an art project where I am attempting to use Fritz Haber's methane whistle in conjunction with the gas emitted by methanogens. I'm funding the project myself, and most anaerobe jars are expensive from the point of view of what I have available to spend on the project.
To a novices eye it looks like an anaerobic jar could be prepared using a mason jar, but then I read that the lid of an anaerobic jar requires a certain palladium catalystic element in order to induce the conversion of O2 and hydrogen to water.
I was therefore wondering if anyone had heard of anyone who created an anaerobic jar, in DIY fashion?
Stephen
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