What's the rule here: Does a patent expire 20 years from date of issue,
or of application? Many patents are applied for years before they are
published, so an "Invention"* may be have been issued a patent 5 years
after application.
Don't complain about searching US patents, btw. You guys have it
insanely easy compared to the EU patent office(s!!!). You have Google
Patents, Patentstorm and heaps of other organisations to make your
searching easy.. we have one atrocious clearing-house with awful, awful
software, often without any supporting documentation besides the title
of a patent, and to get any real information you're then directed to
individual country patent offices.
Oh, and EU patents are often applied for or granted years after the US
equivalents, apparently without backdating to the supposed time of
"Invention"*.
*Where "Invention" means "DNA I was luckily the first to read, and stole
from you all for two decades, kthx".
On 12/03/12 11:57, Darrell Montana wrote:
> Serhat -
>
> You can also search a uspt.gov.. (link here
> http://patft.uspto.gov/netahtml/PTO/search-adv.htm)
>
> In the box use the query below:
>
> *ABST/(restriction AND endonuclease)*
>
> You will get a list of all US patents that contain restriction and
> endonuclease in the abstract. Look for the dates the patents were filed.
> All patents filed 20 years ago or longer are now expired and anyone is free
> to reproduce the work. Below is a patent from NEB for production of the
> very useful restriction enzyme NdeI. The patent expired in 2008. Good
> luck
>
> http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&u=%2Fnetahtml%2FPTO%2Fsearch-adv.htm&r=252&f=G&l=50&d=PTXT&s1=%28restriction+AND+endonuclease%29.ABTX.&p=6&OS=abst/%28restriction+and+endonuclease%29&RS=ABST/%28restriction+AND+endonuclease%29
>
>
>
> On Mon, Mar 12, 2012 at 2:36 AM, Serhat Sevli <erke.bilig@gmail.com> wrote:
>
>> When I looked via Google
>>
>> "restriction enzyme patent expire"
>>
>> there are lots of production methods that are expired.
>>
>> is it already forbidden to produce by other companies or DIY
>>
>> 11 Mart 2012 01:16 tarihinde Cathal Garvey <cathalgarvey@gmail.com> yazdı:
>>
>> When I looked up ice nucleation proteins on google patents I found some
>>> worrying looking patents. Universities don't usually worry about patents,
>>> so I'm not sure how seriously to take the implied suggestion that ice
>>> proteins can be "Used by anyone". Still, would value more expert opinion
>>> there: are ice proteins unencumbered by patents?
>>>
>>> Mac Cowell <mac@diybio.org> wrote:
>>>
>>>> Would ice-affinity purification work? You link an anti-freeze protein
>>>> moiety to your protein of interest, express, lyse, and expose the
>>>> lysate to a probe that is slowly and evenly cooled (?) to encourage ice
>>>> nucleation. At some point afterwards the AFP is cleaved off.
>>>>
>>>> Overview: http://2011.igem.org/Team:Yale/Protein
>>>>
>>>> The take team has a poster with more info.
>>>>
>>>> Mac
>>>>
>>>> 231.313.9062 // @100ideas // sent from my rotary phone
>>>>
>>>> On Mar 8, 2012, at 4:45 PM, Darrell Montana <dmontan99669@gmail.com>
>>>> wrote:
>>>>
>>>>> Just a heads up on making restrictions enzymes... If you are going
>>>> to try this you need to co-express the complementary methylase. If
>>>> not, the RE will chop up the coli genome and kill the host...
>>>>>
>>>>>
>>>>>
>>>>> On Thu, Mar 8, 2012 at 11:45 AM, Derek <derekja@gmail.com> wrote:
>>>>> We started to put together such a project up here at the University
>>>> of
>>>>> Victoria for an igem team, but ultimately decided to do something
>>>> else
>>>>> because of the difficulty of the purification problem. Taq is pretty
>>>>> easy since it is thermostable, but restriction enzymes need to be
>>>>> purified in some way. Some of the earlier restriction enzymes are off
>>>>> patent now, so depending on what you want that may not be as much of
>>>>> an issue.
>>>>>
>>>>> BTW. not an entirely ethical way of getting your plasmid (and one
>>>> that
>>>>> would likely hit patent restrictions), but one that sometimes works,
>>>>> is to take a commercial enzyme and use it as the source of
>>>>> transformation DNA. Make sure you use high competency cells for this
>>>>> because there's not much DNA there, but as good as the commercial
>>>>> purification strategies are they often leave enough plasmid DNA in
>>>> the
>>>>> mix to transform with. You still need to figure out what antibiotic
>>>>> they used and reverse-engineer the purification strategy.
>>>>>
>>>>> --Derek
>>>>>
>>>>> On Mar 8, 11:11 am, Ethan <argentu...@gmail.com> wrote:
>>>>>> Forgive me if this has already been discussed, but what is the
>>>> prevailing
>>>>>> opinion on open-source enzymes. For doing DIY plasmid engineering,
>>>> the most
>>>>>> costly part is probably the restriction enzymes and ligase. Any
>>>> kind of
>>>>>> genetic manipulation (PCR, etc) gets expensive as a result of
>>>> enzyme
>>>>>> prices. Do you think it would be possible to develop some sort of
>>>>>> open-source production method for useful enzymes (ie. open source
>>>> vectors
>>>>>> for expressing the enzymes and some easy way to purify them)? This
>>>> is all
>>>>>> just speculation, and my research into the subject has been
>>>> limited. My
>>>>>> experience in protein purification is also quite narrow.
>>>>>>
>>>>>> I am aware that Cathal has an open-source plasmid vector in the
>>>> works. If
>>>>>> there is an effort to create open restriction enzymes, it would be
>>>>>> convenient to coordinate them with the restriction sites on the
>>>> plasmid. I
>>>>>> am just looking for input on all aspects of such a project. What
>>>> are the
>>>>>> legal restrictions with gene patents? Do you think that it is
>>>> viable to
>>>>>> have some sort of chromatography set up that is reasonable for a
>>>> large
>>>>>> number of DIYers to have access too for purifying the proteins
>>>> perhaps via
>>>>>> a common tag? What do you think the costs (and benefits) of an
>>>> endeavor
>>>>>> like this might be? Thanks for your thoughts!
>>>>>>
>>>>>> -Ethan
>>>>>
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>>>
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>>
>>
>> --
>> *Serhat Sevli, MSc*
>> *Genetikçi (Geneticist)
>>
>> +90-535-628-7887
>> erke.bilig@gmail.com*
>>
>>
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>>
>
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