On Thu, Mar 8, 2012 at 10:20 AM, Mega <masterstorm123@gmail.com> wrote:
> So I make Lb-amp agar and plate the lawn on it. at university I was
> shown to autoclave the agar before pouring plates. i can either do
> that in the pressure cooker or just leave it in the microwave for 10
> mins (?)
>
For LB-agar, I boil some water in the flask in the microwave, which is
2 to 3 minutes. That kills anything in there. Then I let it cool and
add my purified H20 and LB-Agar, and boil in microwave until it is all
dissolved.
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