Also make sure that your strain has not developed amp resistance due to exposure/mutation of plasmid.
--Avery
On Thu, Mar 22, 2012 at 11:06 AM, Derek <derekja@gmail.com> wrote:
Amp is not stable at higher temperatures. Make sure you're waiting for your LB-agar to cool to almost gelling temperatures before adding it, quickly mixing, and pouring your plates. Check out http://openwetware.org/wiki/Ampicillin for more details...
--DerekTo view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/3MElOXD1qVYJ.
On Thursday, 22 March 2012 04:19:42 UTC-7, Mega wrote:You know what?--
I did several tries with the ampicilling in different concentrations and can conclude:
The amp does not work because there is a lawn of bacteria on each plate!!!!
I'll try again with 500mg of amp-powder. Maybe it's just far too weak. Hopefully.
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