[DIYbio] DIYBIO Projects This Summer

Hello all,

At the meeting today we discussed some projects that people want to do this summer.  Some of these will be longer and class-style, others will be more of community projects and some will be hackathon/event days.  The difference is that the classes will have some kind of fixed fee associated with them, and will be offered regularly, while the projects will be whatever people bring in/contribute.  I have typed up a list below, along with with a tentative schedule.  Once means we will do it once, and it should be either too hard/tedious to do again, or will only be done again if people are really interested.  Monthly means it will be offered once a month, and start ASAP means that we should start planning now and start when ready.

I figured the best way to keep track of who wants to do what is to make a form and have people add themselves: here

Send this to your friends/ coworkers/ possibly interested people.  The more the better!

Class style:

(Mandatory) Safety Day: Monthly/2x monthly/as needed : $5
Learn important things, like where trash goes, what you can and cannot use, and how to prevent Bad Stuff from happening.  You will operate the autoclave, be introduced to safely using the centrifuge and vortexer, and learn where equipment lives.

GFP Project aka Biotech 101: Monthly : will look into, probably $150-200
A four part class.  Day one covers "Safety Day", and introduces aseptic technique.  Learn to pipette properly, and use the autoclave to make various kinds of media, and then prepare streak plates of e. coli.  Day two will explain the "central dogma" of biology, and we will proceed with transforming e. coli from last time with a plasmid that is TBD.  On day three we will extract plasmid DNA from the bacteria.  Day four will be one of two things: either a protein extraction OR running the extracted plasmid in a gel and visualizing it.

Ginger Beer Day: Once : $5-10, bring your own 2L bottle
Learn to make ginger beer!  Not particularly actually alcoholic, but possibly not suitable for minors.  Fizzy and refreshing, provided you don't let it get weird tasting.

Kombucha Project: Once : $5-10
Learn to make Kombucha (fermented tea).  Technically non-alcoholic, suitable for minors.  Second fermentation turns it into a fizzy, refreshing beverage.

Yoghurt Project: Once : ???
Make yoghurt.  Not 100% on this one--let me know if you want to take lead on this.

Project style:

Grow photosynthetic sea slugs: Start ASAP
Exactly what is says on the box!  Lets get together and figure out how to do this!

Grow Chitons: Start ASAP
Similar to sea slugs/snails.  An ancient creature with 8 armored plates on its back that munches on algae.  These guys would probably get along!

Synthetic Biology Project: Start ASAP
Our most ambitions project.  Lets make a serious dent in synthetic biology research/make an awesome system!

GFP Fish: Start ASAP
We would need to figure out how to source materials for this, but the protocol/results are not out of reach.

Bioluminescent Algae: Once
Grow up a good bioluminescent pet!  These don't smell, they don't bite, and as far as I know, they are non-toxic.

Bioluminescent bacteria from squid/environmental bacteria: Start ASAP
Isolate a novel/useful bacteria from the environment.  Bioluminescent bacteria are definitely an option!

Events/Hackathons:

Build an Incubator!  Intro arduino.  (may)

Microscopy day! (lasers, real microscopes, diy webcam-o-scopes!) (June?)

Sensor hacking day (Build sweet sensors for monitoring biology!) (July?)

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