the growth vs temperature graph (attached to this email).
Note that the difference in growth rate between 37 and 41 degrees Celsius
is almost negligible (it is a log scale, so that is magnified, but growth is a
logarithmic process). So while you might want to avoid temperatures above
45 or below 30, inside of 37 to 41 things are pretty flat.
It falls off steeply below 21 and above 45. But it looks like any 1 degree
difference in the 37 to 41 range is going to look like noise. Remember that
the protocols we've been looking at specify 37 degrees, while the optimum
growth rate happens two degrees above that. It looks like someone picked
37 degrees because it is 98.6 Fahrenheit, the 'normal' human body temperature,
rather than the optimal temperature for the organism.
Most of this is moot, however, as I would bet the internal temperature
sensor in the MSP430 is probably good to 0.1 degree differences all by itself.
But unlike the LM34 and LM35, it needs to be calibrated.
As for those latter chips, I can't see any need for calibrating them.
The physics of voltage references and diode response to temperature don't
change, and the laser trimmed resistors don't age, so I don't see what would
change over time.
-----
Get a free science project every week! "http://scitoys.com/newsletter.html"On Wed, Apr 25, 2012 at 11:10 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Wed, Apr 25, 2012 at 1:31 PM, Simon Quellen Field <sfield@scitoys.com> wrote:Hmm, I see, so temp is direct for chemistry or atoms, but not for the
> A proxy is where you count on a relation to use one easily measured thing
> instead of
> what you actually want to measure. Temperature can be used as a proxy for
> growth only because in a limited range, it correlates with growth. But that
> range needs
> to be known for each organism, and needs to be stated in the protocol, and
> it usually
> isn't.
>
> Clearly temperature is not linear with growth when you reach the heat shock
> levels, or the boiling point. But the growth curve with respect to
> temperature is
> a curve, and we pretend it is linear by taking our samples from a narrow
> range.
>
abstraction of "life" or growth thereof
Also the second link doesn't work
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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